Enzymes 2 Flashcards
What is a Cofactor?
non-protein factors which are essential for enzyme activity.
What is a Coenzyme?
Carbon containing cofactors, temporarily bound to enzyme, derived from water soluble vitamins. Take part in catalysis.
Holoenzyme
With prosthetic group
Aponenzyme
Without prosthetic group
What is Enzyme kinetics?
Study of rates of catalysed reactions. Gives insight into mechanisms of action of enzymes and its role int he cell.
What is steady state kinetics?
The state reached a short time after the initial mixing of an enzyme and substrate.
How is colour change monitored?
Spectrophotometer
How is glucose measured?
Harding’s test
What factors effect enzyme rate?
Conc of enzyme
Conc of substrate
Temperature
pH
The higher the Km the…
Higher the substrate needed to reach Vmax
Why does increase temp increase rate of reaction?
Because it increases the internal mobility - formation of the transition state complex.
What are Isozymes?
They adapt to change in temp and produce different enzymes.
