Enzymes Flashcards

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1
Q

What is metabolism

A

The chemical reactions that occur in the cells of living organisms.

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2
Q

The maintenance of stable conditions in our bodies is called…

A

homestasis

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3
Q

name 2 types of metabolic reactions that occur in our bodies.

A

Anabolic and catabolic

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4
Q

What are enzymes?

A

Enzymes are catalysts made of protein, which speed up a reaction without being used up themselves.

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5
Q

What shape are enzymes most of the time?

A

globular

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6
Q

What factor determines what type of molecule an enzyme will react on?

A

-Each enzyme has a specific shape that will only fit the molecules it works on.
-The shape determines the type of molecule it will react with

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7
Q

Whats a substrate?

A

A substrate is the substance that an enzyme acts on.

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8
Q

Give an example of an anabolic enzyme? Explain why your chosen enzyme is anabolic?

A

1.DNA Polymerase is an enzyme that forms and repairs DNA.
2. It is an anabolic enzyme because it converts simple molecules into more complex molecules.

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9
Q

Give an example of an catabolic enzyme? Explain why your chosen enzyme is catabolic?

A

1.Amylase is a catabolic enzyme that converts startch into maltose.
2. It is a catabolic enzyme because it breaks the substance down into simpler parts.

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10
Q

Where is amylase produced?

A

In the salivary glands and by the panreas.

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11
Q

When enzymes and substrates come together they form an …

A

…enzyme-substrate complex.

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12
Q

Are enzyme reactions reversible?

A

yes e.g. molecule X can be broken into Y and Z, but you can also combine Y and Z into X

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13
Q

Whats an inhibitor?

A

Inhibitors attach to enzymes and destroy their shape. When this happens, they are said to be denatured.

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14
Q

give an example of an inhibitor

A

-Cyanide denatures an enzyme involved in respiration.
-Antibiotics can affect enzymes in bacteria causing them to die.

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15
Q

What does denatured mean?

A

When the shape of an enzyme is lost fully, it is said to be denatured. This is usually a permanent condition.

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16
Q

What is the optimum temp and pH for enzymes?

A

25°C and pH 7

17
Q

Name an enzyme that works better in an acidic pH

A

Pepsin, found in the stomach, works best at pH 2

18
Q

What is bioprocessing? What can it be used to produce?

A

Bioprocessing is the use of enzyme controlled reactions to produce a product. It is used in the production of beer and cheese.

19
Q

Name 3 physical methods of immobilisation?

A

-Absorption ( physically attached to something)
-Membrane
-Trapped in gel

20
Q

Name 2 chemical methods of immobilisation?

A

-Chemically bonded to support
-Chemically bonded to each other.

21
Q

Name 3 advantages of immobilised enzymes.

A

Immobilised enzymes….
-can be easily recovered from the product so you can get a pure sample of the product easily.
-can be reused to cut costs
-become more stable, making less likely for them to become denatured.