Enzymes Flashcards
Temperature on enzyme activity
If temperature too low there is not enough energy to make reactions occur fast enough. The optimum for most human enzymes is 37°C. Over 37°C changes the shape of the enzyme’s active site so the enzyme is now unable to bind to the substrate/ has been denatured. Therefore rate of reaction decreases/ stops.
pH on enzyme activity
Optimum pH is around 7 for most enzymes in the human body however some have specific optimum pH (eg. pepsin with pH 2 to 3). pH either side of the optimum (too high or too low) changes the shape of the enzyme’s active site so the enzyme is now unable to bind to the substrate/ has been denatured and so rate of reaction decreases/ stops.
Substrate/ enzyme concentration on enzyme activity
Higher concentrations of enzymes/ substrate leads to faster reactions due to more collisions until all enzymes being used.
Enzymes
The enzyme and substrate have complementary shapes so the specific substrate fits into enzyme’s active site, forming an enzyme substrate complex. Enzymes break/ form chemical bonds, causing a reaction to occur. Products are made and leave the enzyme.
Rate of reaction calculation
1 / time (s)