ENZYMES Flashcards
Metabolism
All the organism chemical processes including anabolic and catabolic reactions
Anabolic reactions
Building up of molecules (rot in synthesis )
Catabolic reactions
Breaking molecules down (digestion)
Enzymes
A biological protein catalyst made by cells that alter the rate of chemical reactions
Properties of enzymes
-they speed up reactions
-the are not used up
-they do not change
-a high turn over number
Protein structure of an enzyme
Enzymes are rotting with a tertiary structure but have a globular shape . Contain hydrophilic r groups, disulphide bridges , hydrogen bonds .
Active site
Specific 3d shape that binds to an enzymes .
Extracellular
Some enzymes are secreted by exocytosis and catalyse extracellular reactions . Amylase made in the salivary glands move down salivary ducts ,.
Intracellular in solution
Intracellular enzymes act in solution inside cells . Enzymes in solution in the stroma of chloroplasts .
Intracellular membrane bound
Intracellular enzyme may be attached to membranes . In the Cristal of mitochondria they transfer electrons and hydrogen ions in ATP .
Enzyme substrate complex
Intermediate structure formed during an enzyme catalysed reaction where the substrate and enzyme bind temporarily
What is the lock and key theory
The lock and key theory is when A unique active site shape can catalyse only one type of reaction . It ensures enzyme specificity .
Difference between lock and key theory and induced fit theory
The lock and key theory is based on the substrate fitting the active site however the induced fit theory is based on the active site changing shape in order to fit the substrate .
What is the induced fit theory
Induced fit theory is the change in shape of the active site of an enzyme induced by the entry of the substrate .
Lysozyme
-anti bacterial enzyme in human saliva, mucus an tears
-the active site is a groove and sugars on the bacterial cell wall fit into it
-the groove closes over the sugars and lysozyme molecles change shape and hydrolysis the ones
- the cell wall is then weakened