Enzyme mechanics Flashcards
what are the 4 catalytic strategies
covalent
general acid base
metal ion
by appx and orientation
can more than one mech be used by a single enzyme
yes
Covalent catalysis
The active site contains a reactive group that becomes temp covalently modified
gneral acid-base catalysis
a molecule othen than water plays the role of proton f=donor or accepor
metal ions function 3 ways
- stabilize a neg charge on a rxn intermediate
- generate a nucleophile by ncreasing acidity of a nearby molecule (water)
- increae binding energy through interaction w the substrate
approximation and orientation
two S are brought in close proximity and correct orientation when bound to enzyme
enxymes increase with temp until
they are denatured
what is the optimal ph for enzymatic activity
thepoptimal ph varies and reflects the environemnt
how does the ph influence enzyatic activity?
chancges the aa termial
how do inhibitor exist in nature
toxins posions regulators
Inhibitors can be reversible and
irreversible
what are the 3 types of erversible enzyme inbitors
competititive
uncompetitive
noncompetetive
difference bw un and non competitive
uncomp inly binds the ES complex whereas the non binds the enzyme alone or the ES complex
what tiype of effectdoes competitive inhibuution have on vmax and km
increases km byt has no effect on vmac
what type of affect does uncompetitive have
both km and vmax decrease
