Enzyme Kinetics

Question 1

Psuedo first order

Answer 1

If the reaction has a low conc it will not be diependent on it

Question 2

Zero order rxn

Answer 2

Does not depend on [] of reactants

Question 3

What are the four orders of rxns

Answer 3

zero order
first order
pseuod first order
second order

Question 4

How do enzymes increse the reaction rates

Answer 4

decrease the Ea by lowering the TS by increasing the collisions bw molecules

Question 5

Initial velocity V0

Answer 5

moles of product when time is zero

Question 6

Michealis constant

Answer 6

Km tells us the [] of substrate at 1/2 Vmax

Question 7

Pre-steady state

Answer 7

initial mixing of E + S while [ES] builds up

Question 8

Steady state

Answer 8

[ES] is constant

Question 9

Constant [ES]

Answer 9

rate of formation = rate of ES breakdown

Question 10

What is Vmax directly dependent on

Answer 10

Enxyme []

Question 11

Vmax can only be obtained when

Answer 11

all of the enzyme is bound to substrate so ES =Et

Question 12

Km is the concentration of substrate which permists the enzyme to achieve

Answer 12

half Vmax

Question 13

What does Km reflect

Answer 13

The affinity of an enzyme for its substrate

Question 14

what type of affinity does an enzyme with low Km have

Answer 14

High affinity

Question 15

What affinity does a high Km have

Answer 15

low affinity for its substrate

Question 16

what does Km depend on?

Answer 16

substrate
ph
temp
ionic strength