Translation

Question 1

Describe the flow of info in the Central Dogma

Answer 1

DNA - mRNA - protein

Question 2

What is formed by deamination of adenosine

Answer 2

inosine

Question 3

Wobble base pairing

Answer 3

some tRNAs recognize more than one codon

Question 4

The formation of a peptide bond b/w free aa is thermodynamically unfavourable so what activated intermediates do we need for protein syntheis?

Answer 4

amino acyl tRNA

Question 5

What are the two steps in tRNA charging

Answer 5

1. the aminoacyl-AMP is transferred to tRNA

2. Adenylation and transfer is catlayzed by amino acyl tRNA sythtase

Question 6

Polycistronic

Answer 6

a single strand that can be encoded for many different proteins

Question 7

What is the shine dalgerno seq

Answer 7

Upstream of the start codon ribosmal bidnign site

Question 8

What are some post-translational protein modifications

Answer 8

aa side chains that are most commonly modifed have hydroxy, amino, or thiol functional groups

Question 9

tRNA

Answer 9

adaptor molecules b/w nucleic acid and protein

Question 10

How are aa activated and linked to a specific tRNA

Answer 10

by aminoacyl-tRNA sythetase

Question 11

ribsome

Answer 11

a large complex of protein and RNA

Question 12

Proteins are modified after translation

Answer 12

Key functional groups are commonly covalently modified to alter cheisty and protein function