Enzyme kinetics 2 Flashcards
allosteric enzymes
regulate the flux of biochemicals through metabolic pathways
What can allsoteric enzymes recognize
inhibuoroy and stimulatory molecules
Do allosteric enzymes folllow MM kinetics?
No
Related form (R)
catalyzes rxns
Tense form (T)
less active but more stable and more common
Allosteric constant
T/R-L0
symmetry rule
all active site must be in the same state
what does substrate bind more readilly R or T
R
is the tense form more or less active
less
What happens when [S] increases
it binds an active site on r and all the enzymes become R bc of the symmetry rule
cooperativity
the enzyme disrupts the t-R equil. in favour of R
Threshold effect
allosteric enzymes are more senstive to changes to [s] near KM than MM enzymes with the same vmax
How do regulators modulate allosteric enzymes
mudulate the T-R equilibrium
Heterotropic efffect
regulators shift sigmoidial curve left ot right
homotropic effect
substrate effect omn curve
