Enzyme Kinetics - Ekun Flashcards
The biochemical reaction which an enzyme catalyzes is based on
Primary, secondary, tertiary and quaternary structure of the enzyme
What gives enzymes a secondary structure
Turning and bending of polypeptide chain
What are enzymes
Enzymes are proteins that catalyze a biochemical reaction without altering the equilibrium point of the reaction and remain unchanged and not consumed by the reaction
What are isoenzymes
The same enzymes in multiple form catalyzing the same reaction. It may differ in kinetic properties, structure and tissue distribution
What are isoforms
Variation in proteins produced from the same or related genes through processes like post translational modification of genes.
It differs in structure, regulation or activity
What are cofactors
They are non-protein organic or inorganic molecules that aid in enzymes reactions
Inorganic (conezymes)
Organic (metal enzyme activator)
What is an apoenzyme
Inactive protein portion of the enzyme
Apoenzyme + coenzyme
Haloenzyme
What are coenzymes
They are loosely bound organic molecules that assist in catalyzing enzyme reactions
What are prosthetic groups
Tightly bound coenzymes/organic cofactor with the enzymes
What is enzyme kinetics
It is a branch of enzymology that studies the rate at which enzyme catalyzed reactions occur and the factors influencing these rates
Is michealis- menten constant and michaelis menten model the same?
Yes or no (give reason)
No it isn’t
The constant is the km (enzymes affinity for a substrate)
The model is a measure of the substrate concentration and the rate of enzymes catalyzed reactions
Enzyme substrate formation is reversible
TRUE/FALSE
True
Product formation is reversible
TRUE/ FALSE
False
What is the first order kinetics
The rate of reaction of enzyme with the substrate (enzyme substrate complex) is proportional to the concentration of substrate
The graph of rate of reaction against substrate concentration is what shape
a rectangular hyperbola.
What is zero order kinetics
This is a point when all reactions are independent on the substrate concentration
What is a more useful representation of michaelis menten kinetics
Libeweaver-burk plot
What is the use of lineweaver-burk plot
This plot helps in determining the type of enzyme inhibition by showing how inhibitors affect the enzyme’s Km and Vmax.
Is the effect of enzyme concentration proportional to the rate of reaction
TRUE/FALSE
True
What is Vmax
maximum velocity or velocity of reaction (V) is the number of substrate molecules converted to product per unit time
What is the unit for vmax
µmol/min
What happens when the enzyme digresses from its normal ph?
Slow or denatures enzyme activity
What is the optimum phonon the blood
7-8
What is the optimal ph of pepsin enzyme
1.5
What is the optimal PH of ALP enzyme
10.5
