Enzyme kinetics Flashcards
Why does Km not change during non-competitive inhibition?
Active site is not competed for by he inhibitor
How do you calculate Kcat?
Vmax/Enzymeconc = Kcat
What is the kcat constant?
The number of substrates an enzyme can process per unit time
What would the lineweaver-burk plot for a competitively inhibited enzyme look like?
What would the lineweaver-burk plot for a non-competitively inhibited enzyme look like?
In competitive inhibition, what happens to Km and Vmax?
Km increases
Vmax stays the same
What is the beer-lambert law?
In non-competitive inhibition, what happens to Km and Vmax?
Km stays same
Vmax decreases
What does the x-intercept on a lineweaver-burk plot represent?
What does the y-intercept on a lineweaver-burk plot represent?
What does a lineweaver-burk plot slope represent?
What does Km represent?
The concentration of a substrate where the enzyme reaction velocity is exactly 50%
