Enzyme Kinetics Flashcards

1
Q

Michaelis menten kinetics: types of reactions

A

1st order reaction

pseudo 1st order reaction

zero order reaction

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2
Q

1st order reaction

pseudo 1st order reaction

zero order reaction

A

1st order reaction: rate = [S], the concentration of a single substrate is directly proportional to the rate of the rxn. linear relationship between [s] and product formation

pseudo 1st order reaction: only one substrate effects the rxn rate. have multiple substrates but one is already fully saturated the enzyme

zero order reaction: the rate stops increasing even though the [S] is increasing because enzymes are saturated

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3
Q

what are the 2 things being measured in michealis-menten equation

A

vmax= maximum rate
km= enzyme affinity

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4
Q

Michaelis-menten equation

A

Vo= Vmax[s] / ([s]+Km)

Vo= initial velocity, initial rate of rxn. When [s] is high and [p] is low there’s no reverse rxn

Vmax= maximum rate/ velocity

Km= enzyme affinity. indicates how well the enzyme binds a given molecule (inverse relationship)
small km= high affinity
large km= low affinity

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5
Q

line weaver-burk

A

reciprocal of Michaelis menten, straight line graph

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6
Q

what are the x intercept and y intercept in line weaver-burk plot

A

x intercept = -1/Km (NEGATIVE)
y intercept= 1/Vmax

higher Vmax is lower y intercept
higher Km, less affinity = largest x value
lowest Km, highest affinity= most negative X value

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