Enzyme Inhibition

Question 1

a reverse inhibitor is a substance that

Answer 1

binds to an enzyme to inhibit it but can be released (non-covalent bonding)

Question 2

competitive inhibitors are molecules that

Answer 2

compete with the substrate for binding at the active site of an enzyme

Question 3

in competitive inhibition, Km

Answer 3

increases

Question 4

in competitive inhibition, Vmax

Answer 4

unchanged

Question 5

competitive inhibition can be overcome by

Answer 5

adding more substrate so that the concentration is high enough so that it can outcompete the inhibitor
reason why Vmax is not effected

Question 6

noncompetitive inhibitors are molecules that

Answer 6

bind at an allosteric site (NOT the active site) and inhibits the enzyme by changing it’s confirmation

Question 7

in noncompetitive inhibition, Km

Answer 7

unchanged

Question 8

in noncompetitive inhibition, Vmax

Answer 8

decreases

Question 9

irreversible inhibition is a

Answer 9

substrate that causes inhibition that cannot be reversed
it usually involves the formation or breaking of covalent bonds to or on the enzyme
tends to be toxic

Question 10

uncompetitive inhibitors are molecules that

Answer 10

cannot be bound to the enzyme until the substrate has bonded itself on the enzyme
inhibitor only binds to an allosteric site

Question 11

in uncompetitive inhibition, Km

Answer 11

decreases

Question 12

in uncompetitive inhibition, Vmax

Answer 12

decreases

Question 13

mixed-type inhibitors are molecules that

Answer 13

can bind to either the unoccupied enzyme or the enzyme-substrate complex

Question 14

in mixed-type inhibition, Km

Answer 14

varies

Question 15

in mixed-type inhibition, Vmax

Answer 15

decreases