Enzyme experiments

Question 1

What four factors are to be considered in experiments measuring the rate of enzyme action?

Answer 1

Temperature, pH, enzyme concentration and substrate concentration

Question 2

In each of these experiments, one factor is something while the other three factors must be what?

Answer 2

One factor must be varied and the other three must be kept constant

Question 3

In varying the 4 factors in enzyme experiments, how is the factor temperature kept constant or varied?

Answer 3

Constant: use water bath(s) at the same temperature

Vary: Use water bath(s) at different temperatures

Question 4

In varying the 4 factors in enzyme experiments, how is the factor pH kept constant or varied?

Answer 4

Constant: Use the same pH buffer

Vary: Use pH buffer of different values

Question 5

In varying the 4 factors in enzyme experiments, how is the factor Enzyme concentration kept constant or varied?

Answer 5

Constant: Add the same volumes of enzyme

Vary: not on the course

Question 6

In varying the 4 factors in enzyme experiments, how is the factor Substrate concentration kept constant or varied?

Answer 6

Constant: Add equal volumes of the same substrate

Vary: Not on the course