ENTEROBACTERIACEAE II

Question 1

Three (3) types

Answer 1

• O or somatic antigen (Outer membrane)
• K or envelope antigen (Vi antigen for Salmonella)
• H or flagellar antigen

Question 2

Heat stable and is the lipopolysaccharide (outer membrane) of the cell envelope.
(3 types)

Answer 2

O or somatic antigen

Question 3

Composes the endotoxin portion.
(3 types)

Answer 3

O or somatic antigen

Question 4

Heat labile and consists of capsular polysaccharide.
(3 types)

Answer 4

K or envelope antigen

Question 5

Vi antigens of Salmonella is categorized?

Answer 5

K antigen

Question 6

Inactivated by heating at 100C for 10 minutes to detect the O antigen.

Answer 6

K - or Capsular antigen

Question 7

Found in motile members.
(3 types)

Answer 7

H or Flagellar antigen

Question 8

Heat labile and used to serotype within species of Salmonella.

Answer 8

H or Flagellar antigen

Question 9

Kauffman-White classification
(3 types)

Answer 9

H or Flagellar antigen

Question 10

Selects gram (-) bacteria and also differentiates lactose fermenters from non-lactose fermenters.

Answer 10

MacConkey

Question 11

Bile salts, neutral red and crystal violet – inhibit gram (+) bacteria

Answer 11

MacConkey

Question 12

Lactose – Carbohydrate source

Answer 12

MacConkey

Question 13

Neutral red – brown in pH 6.8 to 8.0 and pink-red at pH less than 6.8

Answer 13

MacConkey

Question 14

Inhibits normal flora coliforms and also differentiates lactose fermenters from non-lactose fermenters.

Answer 14

Hektoen Enteric Agar

Question 15

Bile salts (high amounts) – inhibit gram (+) bacteria and gram (-)

Answer 15

Hektoen Enteric Agar

Question 16

Lactose and sucrose – Carbohydrate source.

Answer 16

Hektoen Enteric Agar

Question 17

Bromthymol blue – indicator

Answer 17

Hektoen Enteric Agar

Question 18

Sodium thiosulfate – sulfur source

Answer 18

Hektoen Enteric Agar

Question 19

Ferric ammonium Citrate - to detect H2 S production.

Answer 19

Hektoen Enteric Agar

Question 20

Selects for stool pathogens and differentiates xylose fermenters (yellow) from non-xylose fermenters (red)

Answer 20

XLD Agar

Question 21

Sodium deoxycholate – inhibit gram (+), gram (-) coliforms, swarming of Proteus

Answer 21

XLD Agar

Question 22

Xylose – Carbohydrate source

Answer 22

XLD Agar

Question 23

Phenol red – indicator

Answer 23

XLD Agar

Question 24

yellow colonies with black centers

Answer 24

Proteus — XLD Agar

Question 25

red colonies

Answer 25

Shigella — XLD Agar

Question 26

yellow colonies

Answer 26

E.coli — XLD Agar

Question 27

red colonies w/ black centers

Answer 27

Salmonella — XLD Agar

Question 28

Selects for stool pathogens and differentiates lactose fermenters (pink-red) from non-lactose fermenters (clear)

Answer 28

Salmonella-Shigella Agar

Question 29

Also known as Yersinia Selective Agar

Answer 29

CIN Agar (Cefsulodin-Irgasan-Novobiocin)

Question 30

Selective media for Yersinia species

Answer 30

CIN Agar (Cefsulodin-Irgasan-Novobiocin)

Question 31

Enrichment broth for cultivation of GI pathogens

Answer 31

Enrichment Broths

Question 32

With high amount of bile salt to inhibit gram (+) organisms and multiplication of normal flora coliforms.

Answer 32

Enrichment Broths