ENTEROBACTERIACEAE II Flashcards
Three (3) types
- O or somatic antigen (Outer membrane)
- K or envelope antigen (Vi antigen for Salmonella)
- H or flagellar antigen
Heat stable and is the lipopolysaccharide (outer membrane) of the cell envelope.
(3 types)
O or somatic antigen
Composes the endotoxin portion.
(3 types)
O or somatic antigen
Heat labile and consists of capsular polysaccharide.
(3 types)
K or envelope antigen
Vi antigens of Salmonella is categorized?
K antigen
Inactivated by heating at 100C for 10 minutes to detect the O antigen.
K - or Capsular antigen
Found in motile members.
(3 types)
H or Flagellar antigen
Heat labile and used to serotype within species of Salmonella.
H or Flagellar antigen
Kauffman-White classification
(3 types)
H or Flagellar antigen
Selects gram (-) bacteria and also differentiates lactose fermenters from non-lactose fermenters.
MacConkey
Bile salts, neutral red and crystal violet – inhibit gram (+) bacteria
MacConkey
Lactose – Carbohydrate source
MacConkey
Neutral red – brown in pH 6.8 to 8.0 and pink-red at pH less than 6.8
MacConkey
Inhibits normal flora coliforms and also differentiates lactose fermenters from non-lactose fermenters.
Hektoen Enteric Agar
Bile salts (high amounts) – inhibit gram (+) bacteria and gram (-)
Hektoen Enteric Agar
Lactose and sucrose – Carbohydrate source.
Hektoen Enteric Agar
Bromthymol blue – indicator
Hektoen Enteric Agar
Sodium thiosulfate – sulfur source
Hektoen Enteric Agar
Ferric ammonium Citrate - to detect H2 S production.
Hektoen Enteric Agar
Selects for stool pathogens and differentiates xylose fermenters (yellow) from non-xylose fermenters (red)
XLD Agar
Sodium deoxycholate – inhibit gram (+), gram (-) coliforms, swarming of Proteus
XLD Agar
Xylose – Carbohydrate source
XLD Agar
Phenol red – indicator
XLD Agar
yellow colonies with black centers
Proteus — XLD Agar
red colonies
Shigella — XLD Agar
yellow colonies
E.coli — XLD Agar
red colonies w/ black centers
Salmonella — XLD Agar
Selects for stool pathogens and differentiates lactose fermenters (pink-red) from non-lactose fermenters (clear)
Salmonella-Shigella Agar
Also known as Yersinia Selective Agar
CIN Agar (Cefsulodin-Irgasan-Novobiocin)
Selective media for Yersinia species
CIN Agar (Cefsulodin-Irgasan-Novobiocin)
Enrichment broth for cultivation of GI pathogens
Enrichment Broths
With high amount of bile salt to inhibit gram (+) organisms and multiplication of normal flora coliforms.
Enrichment Broths
