Enterics Flashcards
Enteric properties (gram, oxidase, growth, fermentation, reduction, motility)
Gram negative bacilli
Oxidase negative
facultative anaerobes
ferments glucose = acid and gas (aerogenic) OR acid only (anaerogenic)
reduce nitRATES to nitRITES
peritrichous flagella or non motile
Which is the only oxidase pos enteric
Plesiomonas
where are isolation of enterobacters are significant
extraintestinal origin (urine, wound, swabs)
which specimens are specifically examined for enterics
fecal specimens from intestinal disease patients
what is an enteric screen?
combination of differential, selective, and enrichment media chosen to isolate organisms
what organisms lead to acute gastrointestinal disease
bacillus cereus, staph aureus, clostridium perfringins
how does the GI become distressed due to organisms?
intoxication
what is the appearance of salmonella, shigella, and yersinia on MAC
wt
what is salmonella on hektoen
blue-green with black colonies
what is shigella on hektoen
green transparent
what is yersinia on hektoen
blue-green
what does yersinia look on cefsulodin irgasan novobiocin (CIN)
pink-red bulls eyes
what does Ecoli O157:H7 look on SORB/SMac and STEC
colorless on SMac
mauve on STEC
what is the plating for spot indole
BAP/CAP
what is the reagent for kovacs indole
dimethylamino benzaldehyde
hat is the reagent for spot indole
p-dimethylaminocinnaldehdye
purpose of CNA and PEA aerobic incubation?
CNA = GP bacteria isolation (and yeast)
PEA = selects GPC staph and strep species (isolation of facultative anaerobes***)
What 2 conditions are needed for decarboxylation
anaerobic and acidic condition (37 degrees)
why do we need a control tube for decarboxylation
tests if amino acids are used or not by the organism
what is STEC media
qualitative media to detect shiga-like toxin producing Ecoli
when do we use susceptibility testing on enterics
immunocomp and if treatment is not working
when do we report yeast in stool
predominant organism present
what bacteria grows pk on MAC
Ecoli (pink) and O157:H7
Kleb pneumo
Klab oxytoca
Kleb aerogenes
Enterobacter cloacae
Citrobacter frundii
what is the TSI of a pk MAC
A/A g
which bacteria is pk on MAC ONLY with A/A g and H2S
citrobacter freundii
which bacteria is wt on MAC ONLY with A/A g and H2S
proteus vulgaris
what is the TSI of wt on MAC
K/A g
what are the bacteria with wt on MAC
Ecoli (white)
proteus mirabilis
proteus vulgaris
providencia rettgeri
morganella morganii
salmonella
shigella
yersinia
what is the TSI of yersinia
A/A sick (orange bottom, red-yellow top)
what color is serratia marcescens on MAC
cherry red
what does non fermenters mean
aerobic, GNB, that cannot use carbs
are NFB opportunistic agents on debilitated hosts (T/F)
T
where is burkholderia most commonly seen
CF patients
what are the NFB?
pseudomonas, steno, burkolderia, alcaligenes, moraxella lacunata, acintobacter
which NFB shows no grow on MAC
moraxella lacunataa
which NFB is wet mount negative
moraxella lacunata and acinetobacter
what are the neg motile enterics
kleb pnuemo, ocytoca
shigella
yersinia
