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HEMATOLOGY > ENHANCEMENT CLASS LAB: EVACUATED TUBES AND PERIPHERAL BLOOD SMEAR > Flashcards

ENHANCEMENT CLASS LAB: EVACUATED TUBES AND PERIPHERAL BLOOD SMEAR Flashcards

1
Q

Drawing blood from the circulatory system through an incision

A

PHLEBOTOMY

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2
Q

○ To obtain a sample for analysis and diagnosis
○ To monitor levels of blood components
○ To treat blood-related disorders

A

PHLEBOTOMY

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3
Q

○ To obtain a sample for analysis and diagnosis
○ To monitor levels of blood components
○ To treat blood-related disorders

A

PHLEBOTOMY

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4
Q

METHODS OF PHLEBOTOMY

A

● Syringe
● ETS

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5
Q
  • Allow multiple tube collection
  • Pressure plays the role of blood drawing
  • No air contaminant
  • Less possibility of contamination
A

ETS

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6
Q

The bigger the gauge, the smaller the needle/bore

A

Needle gauge

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7
Q

14 gauge color

A

olive

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8
Q

15 gauge color

A

amber

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9
Q

16 gauge color

A

gray

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10
Q

18 gauge color

A

green

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11
Q

20 gauge color

A

pink

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12
Q

21 gauge color

A

purple

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13
Q

22 gauge color

A

blue

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14
Q

23 gauge color

A

orange

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15
Q

25 gauge color

A

red

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16
Q

27 gauge color

A

white

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17
Q

Culture

A

Yellow (5) SPS

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18
Q

PT, APTT, INS

A

Light blue (3-4)

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19
Q

Chemistry, Serology

A

Gold/Tiger (5)

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20
Q

Red plastic inversion

A

5

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21
Q

Red glass inversion

A

none

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22
Q

Clinical Chemistry, Hematology

A

Orange (5-6)

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23
Q

Heparin

A

Green (8-10)

23
Q

Plasma Separator Tube

A

Light green (8-10)

24
Q

(K3 - liquid, glass/ K2 -plastic) Blood banking, Blood donation

A

EDTA (8-10)

25
Q

PPT separator tube, PCR,
Molecular testing

A

Pearl/White (8-10)

26
Q

Fluoride, POtassium oxalate,
sodium chloride

A

Gray (8-10)

27
Q

Proper labelling:

A

name, age sex, time, date,
phlebotomist’s initials

28
Q

● Thin layer of blood smeared on a glassmicroscopic slides and then stain
● Conjunction with CBC, to provide inexpensive but reliable and investigation to a variety of hematological disease

A

PERIPHERAL BLOOD SMEAR (FILM)

29
Q

PERIPHERAL BLOOD SMEAR (FILM)
● Used for

A

○ Morphological evaluation of RBC,
WBC, Platelets, and the younger counterparts
○ WBC differential Count
○ Platelete estimate

30
Q

RBC morphological abnormalities

A

Elliptocyte / Ovalocyte
Spherocyte
Stomatocyte
Schistocyte/Schizocyte
Teardrop cell / Darcocyte
Sickle cell
Echnicocyte / Burr Cell
Acanthocyte / Spurr cell
Target cell / Codocyte

31
Q

Elliptical (cigar-shaped), oval (egg-
shaped) RBC

A

Elliptocyte / Ovalocyte

32
Q

RBC with slit-like area of central pallor

A

Stomatocyte

33
Q

Small, round, dense RBC with no
central pallor

A

Spherocyte

34
Q

Fragmented RBC caused by rupture in the peripheral circulation

A

Schistocyte/Schizocyte

35
Q

RBC with a single pointed extension
resembling a teardrop or pear

A

Teardrop cell / Darcocyte

36
Q

Thin, dense, elongated RBC pointed at each end; may be curved

A

Sickle cell

37
Q

RBC with blunt or pointed, short
projections that are usually evenly
spaced over the surface of cell; present in all fields of blood film but in variable numbers per field

A

Echnicocyte / Burr Cell

38
Q

Small, dense RBC with few irregularly spaced projections of varying length

A

Acanthocyte / Spurr cell

39
Q

RBC with hemoglobin concentrated in the center and around the periphery resembling a target

A

Target cell / Codocyte

40
Q

SMEAR TECHNIQUE TYPES

A

Wedge or Two-slide method
Coverslip method
Automated method

41
Q

Wedge or Two-slide method TYPES

A
  • Push type
  • Pull type (most common)
42
Q

Coverslip method

A

bone marrow testing/aspirates

43
Q

● 2-3mm in diameter
● 30-45 degree
● 3⁄4 across the width of the slide

A

Push-type wedge

44
Q

● Anticoagulated blood
● Multiple slide can be made
● Smears should be made within 4 hours

A

ETHYLENEDIAMINE TETRAACETIC ACID (EDTA)

45
Q

● Finger stick or heel stick blood
● Smears must be made immediately
● Remedy: use EDTA microcollect

A

CAPILLARY BLOOD (Fresh or Heparinized)

46
Q

Wright stain

A

Hematology

47
Q

Gram stain

A

Bacteriology

48
Q

Size of blood

A

● Too large - Too thick
● Too small - Too thin

48
Q

FACTORS IN BLOOD SMEAR

A

Size of blood
Angle
Speed
Pressure

49
Q

Pressure

A

● Increased - Thin smear
● Decreased - Long smear

50
Q

Angle

A

● High angle - Short smear
● Low angle - Long smear

50
Q

Speed

A

● Fast - Short smear
● Slow - Long smear

51
Q

Blood smear must be read in __________

A

monolayer part or c section

52
Q
A

HEMATOLOGY (7 decks)

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