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HEMATOLOGY > ENHANCEMENT CLASS LAB: EVACUATED TUBES AND PERIPHERAL BLOOD SMEAR > Flashcards

ENHANCEMENT CLASS LAB: EVACUATED TUBES AND PERIPHERAL BLOOD SMEAR Flashcards

1
Q

Drawing blood from the circulatory system through an incision

A

PHLEBOTOMY

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2
Q

○ To obtain a sample for analysis and diagnosis
○ To monitor levels of blood components
○ To treat blood-related disorders

A

PHLEBOTOMY

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3
Q

○ To obtain a sample for analysis and diagnosis
○ To monitor levels of blood components
○ To treat blood-related disorders

A

PHLEBOTOMY

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4
Q

METHODS OF PHLEBOTOMY

A

● Syringe
● ETS

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5
Q
  • Allow multiple tube collection
  • Pressure plays the role of blood drawing
  • No air contaminant
  • Less possibility of contamination
A

ETS

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6
Q

The bigger the gauge, the smaller the needle/bore

A

Needle gauge

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7
Q

14 gauge color

A

olive

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8
Q

15 gauge color

A

amber

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9
Q

16 gauge color

A

gray

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10
Q

18 gauge color

A

green

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11
Q

20 gauge color

A

pink

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12
Q

21 gauge color

A

purple

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13
Q

22 gauge color

A

blue

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14
Q

23 gauge color

A

orange

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15
Q

25 gauge color

A

red

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16
Q

27 gauge color

A

white

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17
Q

Culture

A

Yellow (5) SPS

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18
Q

PT, APTT, INS

A

Light blue (3-4)

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19
Q

Chemistry, Serology

A

Gold/Tiger (5)

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20
Q

Red plastic inversion

A

5

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21
Q

Red glass inversion

A

none

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22
Q

Clinical Chemistry, Hematology

A

Orange (5-6)

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23
Q

Heparin

A

Green (8-10)

23
Q

Plasma Separator Tube

A

Light green (8-10)

24
(K3 - liquid, glass/ K2 -plastic) Blood banking, Blood donation
EDTA (8-10)
25
PPT separator tube, PCR, Molecular testing
Pearl/White (8-10)
26
Fluoride, POtassium oxalate, sodium chloride
Gray (8-10)
27
Proper labelling:
name, age sex, time, date, phlebotomist’s initials
28
● Thin layer of blood smeared on a glassmicroscopic slides and then stain ● Conjunction with CBC, to provide inexpensive but reliable and investigation to a variety of hematological disease
PERIPHERAL BLOOD SMEAR (FILM)
29
PERIPHERAL BLOOD SMEAR (FILM) ● Used for
○ Morphological evaluation of RBC, WBC, Platelets, and the younger counterparts ○ WBC differential Count ○ Platelete estimate
30
RBC morphological abnormalities
Elliptocyte / Ovalocyte Spherocyte Stomatocyte Schistocyte/Schizocyte Teardrop cell / Darcocyte Sickle cell Echnicocyte / Burr Cell Acanthocyte / Spurr cell Target cell / Codocyte
31
Elliptical (cigar-shaped), oval (egg- shaped) RBC
Elliptocyte / Ovalocyte
32
RBC with slit-like area of central pallor
Stomatocyte
33
Small, round, dense RBC with no central pallor
Spherocyte
34
Fragmented RBC caused by rupture in the peripheral circulation
Schistocyte/Schizocyte
35
RBC with a single pointed extension resembling a teardrop or pear
Teardrop cell / Darcocyte
36
Thin, dense, elongated RBC pointed at each end; may be curved
Sickle cell
37
RBC with blunt or pointed, short projections that are usually evenly spaced over the surface of cell; present in all fields of blood film but in variable numbers per field
Echnicocyte / Burr Cell
38
Small, dense RBC with few irregularly spaced projections of varying length
Acanthocyte / Spurr cell
39
RBC with hemoglobin concentrated in the center and around the periphery resembling a target
Target cell / Codocyte
40
SMEAR TECHNIQUE TYPES
Wedge or Two-slide method Coverslip method Automated method
41
Wedge or Two-slide method TYPES
- Push type - Pull type (most common)
42
Coverslip method
bone marrow testing/aspirates
43
● 2-3mm in diameter ● 30-45 degree ● 3⁄4 across the width of the slide
Push-type wedge
44
● Anticoagulated blood ● Multiple slide can be made ● Smears should be made within 4 hours
ETHYLENEDIAMINE TETRAACETIC ACID (EDTA)
45
● Finger stick or heel stick blood ● Smears must be made immediately ● Remedy: use EDTA microcollect
CAPILLARY BLOOD (Fresh or Heparinized)
46
Wright stain
Hematology
47
Gram stain
Bacteriology
48
Size of blood
● Too large - Too thick ● Too small - Too thin
48
FACTORS IN BLOOD SMEAR
Size of blood Angle Speed Pressure
49
Pressure
● Increased - Thin smear ● Decreased - Long smear
50
Angle
● High angle - Short smear ● Low angle - Long smear
50
Speed
● Fast - Short smear ● Slow - Long smear
51
Blood smear must be read in __________
monolayer part or c section
52

HEMATOLOGY (7 decks)

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