Energy Metabolism and Krebs Flashcards
Why is ATP hydrolysis gibbs negative?
Reaction is energetically favourable, so releases energy for metabolic work
What is an example of use of Hydrogen donor group from ATP hydrolysis?
Act as a co-enyme in redox e.g. NAD to NADH
Which cells use glucose?
ALL cells use it to produce ATP
Where does glycolysis occur?
In the cytosol of the cytoplasm
How many ATP produced by glycolysis and what is it limited by?
2
PFK1
Glycolysis products?
2 ATP, 2 NADH, 2 pyruvate
What converts glucose to G-6-P
Hexokinase (glucokinase in liver)
Which 2 products is F-1,6-BP converted into?
1) Glyceraldehyde-3-phosphate (2)
2) Dihydroxyacetone phosphate
What happens in Anaerobic Respiration? (4)
1) NAD is regenerated from NADH. 2)Pyruvate, from glycolysis, forms lactate. 3) Lactate dehydrogenase catalyses this reaction.
4) NAD goes back to glycolysis so ATP can be produced.
Regulators of Glycolysis?
1) ATP and AMP
2) Citrate
3) F-1,6-BP
What happens in the link reaction?
Pyruvate + CoA + NAD+ –> ACA + Co2 + NADH + H+
3 rate limiting enzymes of the Krebs cycle?
1) Citrate Synthase
2) Isocitrate dehydrogenase (V Citrate)
3) A-Ketoglutarate dehydrogenase (activated by Ca2+ useful for ATP in ex)
Why does an increase in citrate slow the Krebs cycle?
More citrate, causes CS to be inhibited, which reduces speed of the cycle
