emodepside Flashcards
what is emodepside
resistance breaking
homologue of latrotoxin
what does latrotoxin do
paralyses muscles by triggering neurotransission release
c.elegan
effects of emodepside
slowed development
inhibits pharyngeal pumping
decreases locomotion (forward movement most effected at low conc)
inhibits egg laying
what does inhibition og egg laying lead to
bagging in adult hermaphrodites
what are the 2 gene candidates for encoding latrophillins in c.elegans
lat-1
lat-2
what are the roles of latrophillins
and how has it been investigated
mediating the inhibitory effects of emo on feeding and locomotion
using RNAi ad gene ko
c.elegans
pharyngeal system of Lat-1 mutants
show a reduced sensitivity to EMo
what shows a reduced sensitivity to EMo
lat -1 mutants in the pharyngeal system
c.elegans
locomotion activity at LAT-1 mutant
inhibited in a similar fashion to the wild type
what does lat -2 mutants cause on emo effect
emo doesnt exert its inhibitory effect on locomotion through lat-2 mutants
what doesnt allow emo to exert its inhibitory effect on locomotion
lat -2 mutants
how do the double mutants of lat-1 and 2 respond to emo
similar fashion to the single mutant lat-1
what does the double mutant suggest
clearly emo has latrophillin - independant action
genetic screening of emo on c.elegans muslces what did they use
chemical mutagenesis screening
genetic screening of emo on c.elegans muslces
what is chemical mutagenesis screening
20000 genome for mutants
that could move and propergate on microM emo
which recovered 9 alleles for a single gene Slo-1
what do strains carrying null allele of slo-1 do
resistance to emo on locomotion and feeding
gain of funtion alleles of slo-1 give what
behave similiar to emo treated animals
but are not emo hypersensitive
what is SLo-1
ca activated K+ channel
homologues to mammalian BK channel
slo-1 is also proved by work in what
ascaris muscle which shows a Ca and K dependant hyperpol
c.elegans highly express slo-1 where but not in others
nervous system and body wall muscle but not in
pharyngeal muscles
by expressing slo-1 copy in specific subsets of cells in a slo-1 null background this shows what
that emo can inhibit locomotion activity
when slo-1 is present in body wall muscle or neurones
but can only inhibit feeding if present in neurones
