emodepside

Question 1

what is emodepside

Answer 1

resistance breaking

homologue of latrotoxin

Question 2

what does latrotoxin do

Answer 2

paralyses muscles by triggering neurotransission release

Question 3

c.elegan

effects of emodepside

Answer 3

slowed development
inhibits pharyngeal pumping
decreases locomotion (forward movement most effected at low conc)
inhibits egg laying

Question 4

what does inhibition og egg laying lead to

Answer 4

bagging in adult hermaphrodites

Question 5

what are the 2 gene candidates for encoding latrophillins in c.elegans

Answer 5

lat-1

lat-2

Question 6

what are the roles of latrophillins

and how has it been investigated

Answer 6

mediating the inhibitory effects of emo on feeding and locomotion
using RNAi ad gene ko

Question 7

c.elegans

pharyngeal system of Lat-1 mutants

Answer 7

show a reduced sensitivity to EMo

Question 8

what shows a reduced sensitivity to EMo

Answer 8

lat -1 mutants in the pharyngeal system

Question 9

c.elegans

locomotion activity at LAT-1 mutant

Answer 9

inhibited in a similar fashion to the wild type

Question 10

what does lat -2 mutants cause on emo effect

Answer 10

emo doesnt exert its inhibitory effect on locomotion through lat-2 mutants

Question 11

what doesnt allow emo to exert its inhibitory effect on locomotion

Answer 11

lat -2 mutants

Question 12

how do the double mutants of lat-1 and 2 respond to emo

Answer 12

similar fashion to the single mutant lat-1

Question 13

what does the double mutant suggest

Answer 13

clearly emo has latrophillin - independant action

Question 14

genetic screening of emo on c.elegans muslces what did they use

Answer 14

chemical mutagenesis screening

Question 15

genetic screening of emo on c.elegans muslces

what is chemical mutagenesis screening

Answer 15

20000 genome for mutants
that could move and propergate on microM emo
which recovered 9 alleles for a single gene Slo-1

Question 16

what do strains carrying null allele of slo-1 do

Answer 16

resistance to emo on locomotion and feeding

Question 17

gain of funtion alleles of slo-1 give what

Answer 17

behave similiar to emo treated animals

but are not emo hypersensitive

Question 18

what is SLo-1

Answer 18

ca activated K+ channel

homologues to mammalian BK channel

Question 19

slo-1 is also proved by work in what

Answer 19

ascaris muscle which shows a Ca and K dependant hyperpol

Question 20

c.elegans highly express slo-1 where but not in others

Answer 20

nervous system and body wall muscle but not in

pharyngeal muscles

Question 21

by expressing slo-1 copy in specific subsets of cells in a slo-1 null background this shows what

Answer 21

that emo can inhibit locomotion activity
when slo-1 is present in body wall muscle or neurones
but can only inhibit feeding if present in neurones