ELISA answers

Question 1

What is the full name for ELISA

Answer 1

Enzyme Linked immunosorbent assay

Question 2

what is the main purpose of ELISA

Answer 2

Detect and quantify target antigens

Question 3

what are the four steps of ELISA

Answer 3

1) coating
2) blocking
3) detection
4) Final read

Question 4

what is the purpose of Coating

Answer 4

application of either antigen or antibody to the 96 well-plate to initiate the assay

Question 5

what is the purpose of blocking

Answer 5

to prevent non specific binding

Question 6

what is the purpose of Detection

Answer 6

Applying an antibody to target the antigen of interest

Question 7

what is the purpose of the Final Read

Answer 7

Measurement of the signal produced from the assay for analysis

Question 8

What are the four different types of ELISA

Answer 8

1) direct
2) Indirect
3) Sandwich
4) Competitive

Question 9

Two applications of ELSA

Answer 9

1) Assessment of the immune responses and antibody production in vaccine development and evaluation
2) Quantification of hormones like insulin, cortisol, and thyroid hormones

Question 10

what was the predecessor to ELISA

Answer 10

Radioimmunoassay

Question 11

Two benefits of microfluidic ELISA

Answer 11

1) Cost-effective (reduction of reagent and chemical costs incurred to run the process, 24 minute run time)
2) enhancement of point of care (improved health care outcomes)