electrophysiology Flashcards

1
Q

RMP

A

resting membrane potential

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2
Q

charge of cytoplasm of typical cells

A

-70mV

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3
Q

potassium leak

A

○ Membrane is 50-75x more permeable to K+ that to Na+

§ K+ leak

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4
Q

Gibbs-donnan effect

A

§ Macromolecules assembled inside cells
§ Small building blocks diffuse in, and combine to produce larger molecules (proeteins, RNA) that can diffuse out
§ Most proteins ionise as anions (-ve) plus small ion H+ that can leave the cell
§ Traps negatively charged macromolecules in cytoplasm
§ Significant contribution to RMP

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5
Q

leak channels

A

always open

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6
Q

ligand-gated channels

A

open or shut when bound boy a specific ligand

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7
Q

voltage gated channels

A

open at a specific membrane potential, close at a specific membrane potential

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8
Q

influx of Na causes

A

depolarisation

makes the inside of the cell more positive

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9
Q

efflux of K causes

A

hyperpolarisation

makes the inside of the cell less positive

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10
Q

need for positive feedback

A
  • Passive local potentials diminish over distance so are no good for transmitting messages
    • Depolarising stimuli wont transmit long distances without positive feedback
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11
Q

action potential

A
□ 'all or nothing response'
				□ Always the same size (amplitude)
				□ Positive feedback after threshold value is exceeded 
				□ Des not diminish over distance 
				□ Open voltage gated sodium channels
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12
Q

ligand gated Na channels

A

open when bound by specific ligands

provide initial stimulus

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13
Q

voltage gated sodium channels

A

○ Open when membrane potential voltage exceeds threshold
○ Close a few ms later
○ Inactivated for a few ms afterwards (cant be activated again)

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14
Q
  • Voltage gated channels K+
A

○ Open due to membrane potential voltage exceeding threshold
○ Slower to open that Na channels (open later)
○ Close a few ms later
○ K channels don’t lock

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15
Q

positive feedback in AP depolarisation

A
  • Sub-threshold stimuli cause some Na+ channels to open, some Na+ enters the cell causing further depolarisation
    • Once the threshold membrane potential is exceeded all voltage gated Na channels open
    • Na floods into cell down its electrochemical gradient, causing rapid rise in membrane potential
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16
Q

what causes initial stimulus of depolarisation

A

ligand gated sodium channel

17
Q

depolarisation

A
  • At peak membrane potential, the cell is highly positively charges compared to ECF
    • Once the cell membrane potential peaks, voltage gates Na+ channels close and voltage gated K channels open
    • K+ floods out of the cell down electrochemical gradient causing repolarisation
18
Q

Hyperpolarisation

A
  • After repolarisation, low K+ in cell and Na is being pumped out
    • K+ channels are slower to close so K+ leaks back out of the cell
    • Causes ICF to be more negative than at rest (until all the K+ channels close)
19
Q

Absolute refractory period

A

Neurone cannot be made to reach AP

20
Q

intraceullar calcium

A

maintained at low levels

21
Q
  • Relative refractory period
A

○ Neurone can generate AP, but requires greater stimulus

○ Some Na+ cannels are reactivated

22
Q

After AP, neurones need to recover

A

○ Re-establish polarisation

○ Na+/K+ - ATPase pumps Na out of the cell and K in

23
Q

3 reasons to have a negative RMP

A
  • Asymmetric ion distribution maintained by active transport
    • Membrane is 50-75x more permeable to K+ than Na+ causing K+ leak
    • Gibbs donnan effect
24
Q

a greater stimulus generates

A
  • Greater stimulus does not generate more intense Aps, just more APs
25
action potential propogation
- When an AP is achieved, it is only achieved in a small part of the membrane (not the whole cell) - Each AP generates electrical current, which generates a field, which changes the membrane potential next to the part that has generated an AP - The electrical field depolarises the neighbouring part of the membrane - Phenomenon spreads along the plasma membrane
26
action potentials are spread along
membrane microdomains
27
action potential starts at the
trigger zone - high density of voltage gated sodium channels
28
why can't an action potential propagate backwards
the backward areas have locked channels due to refractory periods
29
AP propagation velocity
2m/s
30
schwaan cells secrete
myelin
31
what does myelin do
fat that insulates and prevents action potentials from occurring
32
myelin secreted by
glial cells schwaan cells in PNS oligodendrocytes in CNS
33
gaps between myeline isolation
nodes of ranvier
34
the process of jumping between nodes of ranvier is called
saltatory conduction