Drugs 2 and membrane

Question 1

Caffeine affecting heart rate of Daphnia practical

Answer 1

Caffeine affecting heart rate of Daphnia practical
IV: concentration of caffeine solution (0.5% or if using testing range of concentrations 0.2,0.4,0.6,0.8,1)
DV:daphnia heart rate measured using a clicker (can see under light microscope) (measure for 1min)
CV: age of Daphnia, size of Daphnia (measured in um under microscope), temperature 20degrees with thermometer. Time to acclimatise (5 mins)using a timer, volume of solution (3cmcubed)
Control: focus on Daphnia in water and measure heart rate with clicker (do this for 1min) repeat this 5 times for mean
Repeat 5 more times and calculate a mean heart rate

Question 2

Why could using daphnia be unethical compared to another animal

Answer 2

Using daphnia would be unethical because the caffeine solution could kill/traumatise daphnia.

Question 3

What are ethical issues using invertebrates

Answer 3

Ethical issues of using invertebrates are:
Invertebrates could possibly feel pain, we don’t know
Caffeine could kill/traumatise invertebrates

Question 4

Ethical issues using invertebrates

Answer 4

Ethical issues using invertebrates
Undeveloped nervous system so may not feel pain
Unethical to use any living organism in procedure
If benefits human health, acceptable
Absolutism promotes use of invertebrates under any circumstances or no circumstances