DNA VIRUSES PART 1: PARVOVIRUS, PAPILLOMAVIRUS, POLYOMAVIRUS

Question 1

All are dsDNA except

Answer 1

Parvovirus

Question 2

All are enveloped except

Answer 2

Parvovirus, Adenovirus, Papovirus

Question 3

All are Icosahedral except

Answer 3

Poxvirus

Question 4

All multiply in nucleus except

Answer 4

Poxvirus

Question 5

GUESS THE VIRUS:
Virion: Icosahedral, 18-26nm in diameter, 32 capsomeres
Composition: DNA (20%), Protein (80%)
Genome: Single-stranded DNA, linear, 5.6kb, MW 1.5-2.0 million
Proteins: One major (VP2) and one minor (VP1)
Envelope: None
Replication: Nucleus, dependent on functions of dividing host cells
Outstanding characteristics:
Environmentally stable
Human pathogen, B19, has tropism for red blood cell progenitors.

Answer 5

Parvovirus B19

Question 6

Protein of Parvovirus B19 for autonomic replication

Answer 6

NS1

Question 7

Receptor for B19

Answer 7

P-antigen (globoside)

Question 8

Co-receptor of P-antigen

Answer 8

Alpha 5 Beta 1 integrins

Question 9

Transmission of Parvovirus B19:

Answer 9

Respiratory secretions
Blood or Blood products
Pregnant women to her baby

Question 10

Parvovirus B19 infection that commonly affect children:

Answer 10

Erythema Infectiosum/ 5th Disease

Question 11

Biphasic illness of Symptomatic infections of children with Erythema Infectiosum (5th Disease)

Answer 11

1. Flu-like symptom
2. Rash (slap cheek appearance)

Question 12

Erythema Infectiosum causes a transient but a persistent rash that lasts for up to _(weeks)

Answer 12

2-3 weeks

Question 13

Erythema Infectiosum in adults causes

Answer 13

Arthralgia and arthritis

Question 14

Parvovirus B19 disease can be severe: complications include

Answer 14

viremia, thrombocytopenia, granulocytopenia, pancytopenia, flu-like symptoms, and congestive heart failure

Question 15

Parvovirus B19 replicates in the _ in _

Answer 15

In the Bone Marrow in erythrocyte precursor cells

Question 16

Incubation Period of Parvovirus B19:

Answer 16

12-18 days

Question 17

Parvovirus B19 infection during pregnancy:

Answer 17

Hydrops fetalis

Question 18

Hydrops fetalis can be managed by

Answer 18

Intra-uterine blood transfusion

Question 19

A novel parvovirus that was described in 2005 and causes a variety of upper and lower respiratory tract illnesses

Answer 19

Human Bocavirus (HBoV)

Question 20

HBoV has been increasingly present as a co-infection with _ and _

Answer 20

Respiratory Syncytial Virus (RSV) and Human Metapneumovirus (hMPV)

Question 21

HBoV infection is highest during the _ months

Answer 21

winter

Question 22

Detection of HBoV has improved with the development of sensitive and specific _

Answer 22

rtPCR assays

Question 23

Treatment for pregnant women with acute parvovirus infection

Answer 23

referred to an obstetrician

Question 24

Treatment for immunocompromised patients and those in aplastic crisis with parvovirus infection

Answer 24

blood transfusion

Question 25

GUESS THE VIRUS: Virion: Icosahedral, 55 nm in diameter Composition: DNA (10%), Protein (90%) Genome: Small Double-stranded DNA, circular, 8 kbp Proteins: Two structural proteins; cellular histones condense DNA in virion Envelope: None Replication: Nucleus Outstanding characteristics: Stimulate cell DNA synthesis Restricted host range and tissue tropism Significant cause of human cancer, especially cervical cancer Viral oncoproteins interact with cellular tumor suppressor proteins

Answer 25

Papillomavirus

Question 26

Envelope proteins of Papillomavirus

Answer 26

E6 and E7

Question 27

Human Papillomavirus Skin warts: Small in size, occur in large numbers anywhere in the body, have roughened surface Complaint: cosmetic appearance Genotypes of HPV: 2-4

Answer 27

Common warts (Verrucae vulgaris)

Question 28

Human Papillomavirus Skin warts: Flat and smooth Generally, affects children Genotype of HPV: 3

Answer 28

Flat warts (Verrucae planae)

Question 29

Human Papillomavirus Skin warts: The appearance of multiple flat lesions all over the body Associated with T-cell deficiency Pre-malignant condition

Answer 29

Epidermodysplasia verruciformis

Question 30

70% due to genotypes 16 and 18 of Human Papillomavirus

Answer 30

Cervical Cancer

Question 31

Signs and Symptoms of Human Papillomavirus during Incubation period

Answer 31

Warts and Cancer

Question 32

Signs and Symptoms of Human Papillomavirus during Infectious period

Answer 32

Lesions

Question 33

Treatment for skin lesions caused by Human Papillomavirus

Answer 33

Liquid nitrogen

Question 34

Treatment for genital lesions caused Human Papillomavirus

Answer 34

Podophyllin

Question 35

Treatment for plantar lesions caused by Human Papillomavirus

Answer 35

Salicylic acid

Question 36

Treatment for systemic infection caused by Human Papillomavirus

Answer 36

Alpha interferon

Question 37

Three vaccines for HPV:

Answer 37

Gardasil Gardasil 9 Cervarix

Question 38

Vaccine for HPV which offers protection against genital warts (genotypes 6 and 11) and cervical carcinoma (genotypes 16, and 18)

Answer 38

Gardasil

Question 39

Vaccine for HPV which protects against two most important oncogenic HPV types (Cervical carcinoma: 16 and 18)

Answer 39

Cervarix

Question 40

Vaccine for HPV effective against throat carcinoma/ oral cancers

Answer 40

Gardasil 9

Question 41

Perinuclear staining in Pap Smears indicative of HPV infection

Answer 41

Koilocytes

Question 42

Polyomavirus and also known as BK virus

Answer 42

Polyomavirus hominis 1

Question 43

Polyomavirus that is a common infection of childhood but specific disease has not been associated with the virus Reactivates in immunocompressed patients

Answer 43

Polyomavirus hominis 1

Question 44

Polyomavirus hominis 1 reactivates in patients who undergo renal transplant and causes

Answer 44

Urethral strictures

Question 45

Polyomavirus hominis 1 reactivates in patients who undergo BM transplant and causes

Answer 45

Hemorrhagic cystitis

Question 46

Treatment for Polyomavirus hominis 1 and the only antiviral agent that may be effective

Answer 46

Cidofovir

Question 47

Laboratory Diagnosis for Polyomavirus hominis 1

Answer 47

PCR

Question 48

Polyomavirus hominis 2 is also know as

Answer 48

JC virus

Question 49

First patient to be diagnosed with Polyomavirus hominis 2

Answer 49

John Cunningham

Question 50

Immunosuppressed/ Immunodeficient patients acquire Polyomavirus hominis 2 due to

Answer 50

T-cell dysfunction

Question 51

Polyomavirus hominis 2 reactivates to cause

Answer 51

Progressive Multifocal Leukoencephalopathy (PML)

Question 52

PML causes

Answer 52

damages the myelin sheath causing change in intellect, affection of motor functions, or sensory loss which eventually leads to death within a year

Question 53

Laboratory Diagnosis for Polyomavirus hominis 2

Answer 53

PCR