DNA synthesis Flashcards
1
Q
What occurs first in the synthesis of the leading strand?
A
DNA primase synthesizes an RNA primer.
2
Q
RNA primer is then extended by?
A
DNA Polymerase 3
3
Q
RNA primers are removed by?
A
DNA polymerase 1
4
Q
DNA fragments on the lagging strand are called?
A
Okazaki fragments
5
Q
What is the difference between leading and lagging strand synthesis?
A
New RNA primers have to be made frequently to allow DNA synthesis to continue
6
Q
What is the role of DNA Polymerase 1?
A
Remove RNA nucleotides of primer from 5’ end and replace it with DNA nucleotides.
7
Q
What is the role of DNA ligase?
A
To join Okazaki fragments of lagging strands; on leading strand, joins 3’ end of DNA that replaces primer to rest of leading strand DNA
