Dna Sequencing Flashcards
1
Q
DNA sequencing
A
determining the bases in a strand of DNA
2
Q
Dna Synthesis in a tube
A
Dna template strand, deoxyribose, primer, dna polymerase
3
Q
Sanger sequencing
A
Combine in a tube
* Primer of known sequence –adjacent to the region of interest
* dNTPs
* Fluorescently labeled ddNTPs
* Many copies of the DNA you want to sequence (template)
* DNA polymerase
* Buffer
