DNA Replication = Interphase Flashcards
Why does DNA need to replicate before cells divide
Each time a new cell is made, the cell must receive an exact copy of the parent cell DNA.
What are the enzymes needed in DNA replication?
- Helicase
- DNA Polymerase
- Primase
- Ligase
What role does the enzyme Helicase play in DNA replication?
Helicase separates double-stranded DNA into single strands allowing each strand to be copied.
What role does the enzyme DNA Polymerase play in DNA replication?
Known as the builder DNA polymerase replicates the DNA for a new strand
What role does the enzyme Primase play in DNA replication?
An initializer primase creates a primer on a DNA strand so that the DNA polymerase knows where to work,
What role does the enzyme Ligase play in DNA replication?
Ligase links the Okazaki fragments together to make one continuous strand of DNA as well as seals repairs and recombination fragments in the DNA.
Okazaki fragments
Small DNA fragments formed during the replication of double-stranded DNA
What is Interphase?
Interphase is the phase of the cell cycle in which a typical cell spends most of its life (90%). It is the ‘daily living’ or metabolic phase of the cell, in which the cell obtains nutrients and metabolizes them, grows, reads its DNA, and conducts other “normal” cell functions. DNA replication takes place here.
What happens during Interphase?
During interphase, cellular organelles double in number, the DNA replicates, and protein synthesis occurs. The chromosomes are not visible and the DNA appears as uncoiled chromatin.