DNA Replication Flashcards
Name the bond between amino acids
Peptide
What additional bond gives secondary structures their shape?
Hydrogen bonds
What is the main shape formed using secondary structure?
Helix
Why is DNA replication important?
Ensures that each new cell receives a full set of chromosomes and therefore no genetic information will have been lost
Describe what is meant by the primary structure of proteins
Order of amino acids
Which two enzymes are involved in DNA replication?
DNA Polymerase and lipase
What is needed to start DNA replication?
RNA Primer
What end of a DNA strand can DNA polymerase add free complimentary DNA nucleotides to?
3’ end
What is the leading strand?
A strand of DNA being replicated continuously
How is the lagging strand replicated?
In fragments
What are the fragments in the lagging strand joined together by?
Ligase
What is mRNA’s job?
Carries genetic code from DNA in nucleus to ribosome
What does rRNA and protein form?
Ribosome
What is tRNA’s job?
Carries a specific amino acid to a ribosome
What is a cell’s genotype determined by?
The proteins that are synthesised when genes are expressed
What does DNA polymerase control on the leading strand?
Sugar phosphate bonding between nucleotides
What is a primer?
A short strand of complimentary nucleotides formed at 3’ end of parental DNA strand
What is it called when replication results in two new DNS molecules? Why is it called this?
Semi conservative replication
Each new DNA molecule receives one strand of original parent molecule
What are 5 things needed to start DNA replication?
DNA (acts as template for new molecule) RNA primer Supply of DNA nucleotides Appropriate enzymes DNA polymerase and lipase
What unwinds the parental double helix?
Helicases
What stabilises the unwound parental DNA?
Single strand binding proteins
What direction is the leading strand synthesised in?
5’>3’
How is an Okazaki fragment formed?
Primate synthesises short RNA primer which is extended by DNA polymerase to form an Okazaki fragment
What happens after the RNA primer is replaced by DNA?
DNA ligase joins the Okazaki fragment to the growing strand
What is a cell’s genotype determined by?
Sequence of the DNA bases in its genetic code
What is a cell’s phenotype determined by?
Proteins that are synthesised when genes are expressed