DNA replication Flashcards

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1
Q
  1. What does the enzyme helicase do during DNA replication?
A

unwinds the double helix structure of DNA, separates to two single strands by breaking the hydrogen bonds between base pairs.

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2
Q
  1. what is a single stranded binding protein?
A

Binds to the DNA strands in order to keep them separated

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3
Q
  1. What does the enzyme primase do in DNA replication?
A

adds RNA primer to each strand, signaling where replication should start.

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4
Q

4.. What is the role of DNA polymerase in DNA replication?

A

-Attaches to primer & adds new nucleotides to original strands
-Builds a new strand by pairing each base with its complementary base.
- leading strand is copied continuously.
-RNA primer is removed after

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5
Q
  1. What happens on the lagging strand during DNA replication?
A

ligase closes gaps creating a complete strand. At sugar phosphate bone

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6
Q

6.What is the end result of DNA replication?

A

2 identical double-stranded DNA molecules are produced, each with one original strand and one newly synthesized strand.

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7
Q

DNA

A

molecule that carries genetic information for all living things.

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8
Q

Template strand

A

single strand of DNA used as a guide to make a new one.

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9
Q

Nucleotides

A

are the building blocks of DNA, made of A, G, C, and T.

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10
Q

Complementary

A

the new strand matches the template strand:
A pairs with T
G pairs with C

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11
Q

direction DNA strands run in

A

opposite directions (ANTI-PARALLEL)

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12
Q

what does DNA replication being semi-conservative mean

A

(one old strand, one new strand

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13
Q

RNA is temporary becuase

A

it needs to be replaced in the end with the final DNA structure

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14
Q

why is it important to replicate DNA

A

in order to fully duplicate cells properly, and to ensure each daughter cell receives a copy of the DNA.

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15
Q

where does DNA replication occur

A

nucleus of eukaryotic cells.

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16
Q

why is proofreading important

A

to prevent errors that could lead to incorrectly coded genes or proteins

17
Q

lagging strand

A

-strand of DNA copied in short pieces.
-opposite to the direction of the replication fork so it takes longer to finish

18
Q
A