DNA & protein synthesis Flashcards

1
Q

What is chromatin?

A

the form of a cel’s dna when it’s not preparing to divide

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2
Q

What is chromatin comprised of?

A

loose strands of dna

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3
Q

what are histones?

A

bead-like protein structures that bunch together

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4
Q

what do you call the bunched up histones?

A

nucleosome

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5
Q

what is wound around histones?

A

DNA

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6
Q

where are histones not found?

A

In bacteria (prokaryotes)

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7
Q

what does chromatin do before mitosis?

A

condenses to form chromosomes

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8
Q

how many strands does DNA have?

A

two

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9
Q

what shape does DNA have?

A

double helix shape

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10
Q

what is DNA made from?

A

building blocks called nucleotides

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11
Q

What are nucleotides comprised of?

A

phosphate group, nitrogenous base, sugar molecule (deoxyribose)

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12
Q

What 4 types of nitrogenous bases in DNA make up the “dna sequence”?

A

Adenine, Thymine, Cytosine, Guanine

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13
Q

What are base pairs connected by?

A

hydrogen bonds

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14
Q

what does adenine (A) pair with?

A

Thymine (T)

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15
Q

What does cytosine (C) pair with?

A

Guanine (G)

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16
Q

What is DNA replication?

A

semiconservative

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17
Q

what does semiconservative mean?

A

the double strand of DNA splits in half and the halves are used as templates for the new strand

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18
Q

What is the first step in dna replication?

A

the dna helix is unwound (untwisted)

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19
Q

What is the second step in dna replication?

A

the double strand is separated, pulling apart the nitrogenous base pairs

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20
Q

What is the third step in dna replication?

A

new nitrogenous bases are laid down on the template strand (AT, CG)

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21
Q

when does dna replication occur in the nucleus?

A

Synthesis phase of interphase (before cell splits in mitosis)

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22
Q

What is the final step in dna replication?

A

two new DNA helices are formed

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22
Q

After replication, how much of the
new DNA is comprised of the original
parent strand?

A

half

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23
Q

what is the purpose of dna?

A

to code for proteins

24
what do proteins do?
control everything in our bodies and give rise to our phenotypes
25
what will mutations in the dna chnage?
the produced protein and phenotype
26
what is the central dogma?
the process of how proteins are made from dna
27
what does the central dogma include?
transcription and translation
27
What are some examples of proteins in the body?
Amylase, pepsin (digestive enzymes)
28
Where does transcription occur?
inside the nucleus
29
what is transcription?
the process of using dna's code to make single-stranded mRNA
29
what do the double strands of dna open up to reveal?
the nitrogenous bases
29
What is mRNA?
It's made as a complimentary code of DNA
29
What does the mRNA do after leaving the nucleus?
attaches to a ribosome in the cytoplasm (or rough ER)
30
what does the ribosome hold?
the mRNA strand
31
What does the tRNA do?
brings amino acid molecules and attaches them to the growing chain
32
what is translation?
the process of building a chain of amino acids form the mRNA template
33
what is a codon?
the 3 bases lined up in the mRNA used as a set
34
what does the tRNA contain?
anticodon
35
what is an anticodon?
a complimentary copy of the codon
36
what happens when the codon and anticodon align?
the tRNA deposits the amino acid it carries onto the growing chain
37
What can an amino acid chain do once it's formed?
it can become a protein
37
what could cause a protein to have the wrong shape?
mutations in the dna
37
what is a connection between mRNA and DNA?
both molecules that carry genetic information
37
what is a protein?
a 3d molecule
38
what is a protein's job based on?
its shape
38
Name 2 differences between mRNA and DNA
dna is double stranded, mRNA is single stranded mRNA doesn't have thymine, but uracil instead
39
What does uracil pair with in mRNA?
Adenine
40
What is a codon chart used for?
to determine the amino acid produced for each codon
41
What do codon charts use?
the mRNA three-letter sequence
42
What is a stop codon?
ends translation so that no new amino acids are added to the growing chain
42
What is a start codon?
it initiates the start of translation
43
Name a start codon
AUG
44
what are mutations?
changes to the dna sequence
45
how do mutations affects the final protein formed?
mutations in the original dna might lead to a wrong amino acid being placed into the amino acid chain. this might cause a shape change in the final protein, disrupting its function
46
will a mutation in the DNA always affect the amino acid chain?
not always. if the mutation still produces the same amino acid, there'll be no difference in the final chain and produced protein
47
what does mRNA do?
carries info from genes
48
what does tRNA do?
transports amino acids to ribosomes for protein synthesis
49
what does rRNA do?
carries out protein synthesis in ribosomes