DNA Organization Flashcards
- Circular chromosomes
- Double stranded (dsDNA)
- May also contain plasmids
- Found in the nucleoid
Organization of DNA in most prokaryotes
Back bone has been nicked.
-relaxes from supercoiling
Relaxed circular DNA
Allows DNA to fit inside the cell
-Amount and type of supercoiling depends on the starting point
Supercoiled Circular DNA
Formation from contortion of supercoiling and twisting.
2 supercoiled DNA forms
Untwisting the DNA
-Compacting DNA
Negative supercoiling
Twisting
- introducing more twisting causes DNA to contort.
- Compacting DNA
Positive supercoiling
Amount and type of supercoiling controlled by:? -highly conserved enzymes -relieve twisting by two types: I. Make a nick in one strand II. Cuts both strands
Topoisomerases
2 DNA linked like a chain
Catenated DNA
How doe bacteria package their DNA?
a. using histones
b. by supercoiling the DNA
c. by acetylating the DNA
d. by methylating the DNA
e. all of the above
b. by supercoiling the DNA
-Nucleus, mitochondria, choloroplasts
Organization of DNA in Eukaryotes
Amount of haploid DNA in an organism in base pairs
C value
Increase in DNA doesn’t always mean and increase in complexity of the organism.
C value paradox
1n
haploid
2n
diploid
Complete set of chromosomes
karyotype
Group that gives DNA its (-) charge
Phosphate