DNA Flashcards

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1
Q

What is replication?

A

DNA is created in a semiconservative process using existing strands as templates. (for cell replication)

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2
Q

What is transcription?

A

RNA is created in the nucleus using DNA strands as templates.

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3
Q

What is translation?

A

ribosomes create proteins using RNA strands as templates.

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4
Q

What is mRNA?

A

contains codon for amino acids

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5
Q

What is tRNA?

A

delivers amino acids to ribosome

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6
Q

What is rRNA?

A

component of the ribsome

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7
Q

Which nucleotides are pyrimidines?

A

C,T

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8
Q

Which nucleotides are purines?

A

G,A

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9
Q

Which nucleotides have 3 H-bonds?

A

C,G

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10
Q

Which nucleotides have 2 H-bonds?

A

A,T

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11
Q

Where does the next nucleotide add in a DNA chain?

A

at the 3’ OH

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12
Q

How are the two chains of DNA held together?

A

H-bonds between nitrogenous bases.

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13
Q

In which direction is DNA read? and by what?

A

3’ to 5’

DNA polymerase

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14
Q

In which direction are nucleotides added?

How is this accomplished in the lagging strand?

A

5’ to 3’

lagging strand (3’ to 5’) is created with (5’ to 3’) okazaki fragments that are later spliced together.

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15
Q

What is involved in post-transcriptional RNA processing?

A

1) 5’ cap is added
2) exons are spliced out
3) 3’ poly-A tail is added

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16
Q

What are the three steps of translation?

A

Initiation: small subunit + tRNA binds to mRNA at start codon; then large subunit joins.

Elongation: new tRNA brings next amino acid; ribosome moves to next codon.

Termination: Ribosome reaches stop codon and releases mRNA and protein.

17
Q

What is the start codon?

A

AUG Methionine

18
Q

What are the stop codons?

A

UAA, UAG, UGA

19
Q

What is the hybridizing character of the N in a peptide bond and why?

A

sp2

the bond between the carbonyl and the nitrogen is partially double-bond due to resonance (N donates lone pair to bond to create negative charge on O).