dna Flashcards
0
Q
3 types of RNA (3)
A
- mRNA (messenger RNA) - single strand that carries the genetic code from nucleus into cytoplasm
- tRNA (transfer RNA)- transports amino acids to ribosomes to assemble proteins
- rRNA (ribosomal RNA)- physical and chemical structure on which protein is actually assembled
1
Q
transcription (4)
A
- code transferring from DNA -> RNA
- occurs in nucleus
- RNA polymerase binds to the DNA at “promoter” and unwinds and separates the helix into 2 strands, one called the sense strand
- as RNA polymerase unwinds the DNA it also adds RNA nucleotides, which are H bonded to DNA
- when RNA polymerase reaches the terminating sequence on the DNA chain it breaks off the DNA
2
Q
DNA replication (3)
A
- both stands in each chromosome are replicated
- DNA polymerase is used, attachs and moves along DNA strand, while DNA ligase causes bonding of successive DNA nucleotides to one another using high E phosphate bonds
- each new DNA strand is attached to a template DNA strand by a hydrogen bond
3
Q
DNA repair (proof reading and mutations) (2)
A
- proofreading: DNA polymerase and ligases proofread the strands and cut defective areas out
- mutations: when a mistake occurs in transcription => abnormal protein; can lead to abnormal cell function and apotosis
4
Q
chromosome replication (4)
A
- human cell has 46 chromosomes (23 pairs)
- DNA is packed into chromosomes and the genes are almost identical
- DNA wrap around (+) charged histones which regulate DNA activity (when tightly wrapped around DNA cant replicate or make RNA)
- chromosomes replicate after DNA replicate
5
Q
cell mitosis nemonic (5)
A
- I (interphase)
- Prefer (prophase)
- Milk (metaphase)
- And (anaphase)
- Tea (telophase)
6
Q
interphase (late) (4)
A
- lengthening of microtubles causing 2 pairs of centrioles to move apart
- at same time other microtubles grow radially away from centriole pairs, forming a spiny star (aster) in the end of each cell
- microtubles between the 2 centrioles = miotic spindle
- microtubles + 2 pairs of centriole = miotic apparatus
7
Q
prophase (and late prophase/ prometaphase) (4)
A
- chromatin condenses -> chromatids
- nucleoli disappear
- prometaphase or late prophase: miotic spindle is growing an punctures nuclear membrane
- microtubles pull one chromatid of each pair towards one pole
8
Q
metaphase (2)
A
- chromosomes cluster at middle of cell
2. centromeres align at equator of spindle
9
Q
anaphase (1)
A
- centromeres split and are pulled to opposite sides of cell
10
Q
telophase (2)
A
- two daughter chromosomes are now pulled apart and miotic apparatus dissolves
- nuclear membrane forms around each set of chromosomes and microfilaments pinch to make two new cells
11
Q
cytokinesis (4)
A
- division of cytoplasm
- begins during late anaphase or early telophase
- plasma membrane over center of cell is drawn inward by contractile filaments and pinched into two daughter cells
- these daughter cells are smaller but identical and will enter interphase and grow