DNA Flashcards
What was already known about genes or what do you know
That they code genetic information and that its a generational molecule/s being passed down. One gene- one polypeptide. Chromosomes were available
Can you explain Griffith experiment with Virulent?
Talk about the 4 different processes with the Mice
Chromosomes are small enough to be transferred but which component, DNA or protein conferred virulence.
Hmmmm
Explain the experiment with RNase. Proase and DNase
Think about what was effected and what was not
What are phages
They are genetic material wrapped in protein. They are viruses that infect bacteria and use them to create more phage. Think about the influenza.
Explain Chargaff’s rule
yes
how is genteic info is stored in chromosomes
think about histones and condensing
Is mitosis started before DNA replication
NO! mitosis cannot occur without DNA replication
Explain why semi conservative is the right way of DNA also explain melelson strahl experiment
COOL
The series of DNA replication
topoisomerasre
helicase
single strand protiens
primase
DNA polymerase
DNA ligase
what is the ori
the segments where DNA gets replicationed multiple sites
The difference between eurkayotic and prokaryotic DNA replication
nice
What is the finishing problem
Where telomerase comes in but why
Errors in disease happen how?
A mutation or inactive enzyme that can change the product
Neurospora mold experiment
Hypothesis - each gene determines on enxyme in a biochemical pathway. Explain the order and what is needed for growth to occur
what does the one gene and one enzyme have to do with mutations?
if an organism cannot convert one particular compund to another. It lacks enzyme required for the next step
explain the central dogma
transcription and translation etc.
protein synthesis isnt always like central dogma …
because of virus. sometimes RNA and code and then go into a polypeptide.
EXPLAIN initiation, elongation and termination.
The promoter, the coding aka the continuation, the release
What editing sequence is removed and which on stays
Introns
Exon
Transcription details
Promoter,RNA polymerase, mRNA, 5cap and poly tail
explain translation
RNA to PROTEIN. relies on gentic code. also think about APE system when making a polypeptide
tRNA
anticodon and amino acid attachment site
tRNA synthase
amino acids charged with ATP. phosphates and bind to their matching tRNA which fuels peptide bond formation.
A site, P site and E site
A site gets the ribosome then P site has the start codon and then E site has the release.
The rest of the process relies on cellular structures of the endomembrane system.
LUMEN, vesicles, packaging etc. But post translation has …
phosphate groups, adding sugar, cleaving polypeptides etc.
remember the 4 levels of protein structures.
primary
secondary
tertiary
Quatendary
