DNA

Question 1

A double helix is

Answer 1

made up of 2 strands of DNA

Question 2

The 4 DNA bases are

Answer 2

Guanine, Cytosine, Adenine, Thymine

Question 3

Adenine pairs with

Answer 3

Thymine

Question 4

Guanine pairs with

Answer 4

Cytosine

Question 5

Deoxyribose sugar can be found at the

Answer 5

3’ end

Question 6

Phosphate can be found at the

Answer 6

5’ end

Question 7

What bonds break after DNA unwinds?

Answer 7

Hydrogen bonds

Question 8

What is needed to start replication

Answer 8

A primer

Question 9

What enzyme joins fragments of DNA together?

Answer 9

Ligase

Question 10

What enzyme adds the complementary nucleotides during replication?

Answer 10

DNA Polymerase

Question 11

What end can nucleotides be added to?

Answer 11

The 3’ end

Question 12

Why is DNA initially heated to 92-98oC during PCR?

Answer 12

To break the hydrogen bonds and separate the strands

Question 13

What doe PCR make?

Answer 13

Amplified copies of DNA

Question 14

During PCR, What temperature is the DNA cooled to and why?

Answer 14

It is cooled to 50-65oC to allow primers to bind

Question 15

What can PCR be used for?

Answer 15

To solve crimes, settle paternity suits and diagnose genetic disorders