Division and Growth

Question 1

Most Bacteria replicate through Binary Fission, but SOME Bacteria and SOME Yeast replicate through _______________ .

Answer 1

Budding

Question 2

The time required for one cell to divide and become two (also the time it takes a population to double)

Answer 2

Generation Time

Question 3

Asexual methods of reproduction (binary fission and budding) allow for very rapid growth. A __________________
is used when talking about bacterial growth to accomodate the large values.

Answer 3

Log Scale

Question 4

What are the 4 distinct phases of growth we see within a culture?

Answer 4

Lag
Log
Stationary
Death

Question 5

This occurs when cells are first inoculated into a culture, and they are adjusting and gearing up for growth

Answer 5

Lag
(minutes to hours or days)

Question 6

This is when cells are actively dividing in exponential format, and at a constant minimum generation time.

Answer 6

Log
(because generation time is constant, the growth curve is a straight line)

Question 7

When nutrients run out in a fixed environment; waste builds up and pH changes causing growth to slow and cells begin dying

Answer 7

Stationary
(divisions = deaths, so curve flattens)

Question 8

Cell deaths overtake the number of new cells being generated.

Answer 8

Death (curve declines)

Question 9

2 Techniques for Plating Growth

Answer 9

1. Pour Plating
2. Spread Plating

Question 10

Direct Microscopic Counts can be performed using a ________________

Answer 10

Cytometer