DFS

Question 1

Indicates Infalammation

Answer 1

Polymorphonuclears (PMNs)

Question 2

Indicate an immune response to a parasitic infection

Answer 2

Eosinophils

Question 3

Indicate ulcerations or bleeding

Answer 3

Red blood cells

Question 4

Usuallya present in both bacterial and parasitic infections.

Answer 4

Macrophages

Question 5

One can mistake the active macrophages for ___

Answer 5

Amebic Trophozoites

Question 6

Are released with the disintegration of eosinophils.

Answer 6

Charcot-Leyden Crystals

Question 7

Plant and animal hairs may look like __

Answer 7

Helminth Larvae

Question 8

__ mg of stool is comminuted thoroughly w/ a drop of __% __

Answer 8

2mg of stool; 0.85% Sodium Chloride solution

Question 9

DFS is primarily useful in the detection of motile __

Answer 9

Protozoan Trophozoites

Question 10

Trophozoites can be stained to demonstrate the nuclear morphology using ___

Answer 10

Nair’s Buffered Methylene Blue solution

Question 11

___ Can also be seein in a DFS

Answer 11

Protozoan Cysts

Question 12

Use ,,, containers with a screw cap

Answer 12

Clean, dry, wide-mouthed, leak-proof

Question 13

__ if liqued
__ if solid

Answer 13

5ml; 20-40g

Question 14

Store at __ and maintain it in cool temperature (inside a
box with coolant) during transport

Answer 14

4 - 8ºC

Question 15

SENDING SPECIMENS TO A REFERENCE
LABORATORY

Preservatives used: ___ (for wet mount
examination) and (for specimens
that require staining)
*
Ratio: __ preservative

Answer 15

10% formalin, polyvinyl alcohol/PVA

3 parts stool to 1 part; 3:1

Question 16

Watery specimens: must be examined ideally within ___ after passage

Soft specimens: must be examined ideally within ___
after passage

Formed specimens: must be examined within ___
after passage

Answer 16

30 minutes

1 hour

12 hours

Question 17

Positive stools are mixed with moistened
soil or granulated charcoal

Answer 17

Copro Culture

Question 18

Harvest larvae using the ___ Technique

Answer 18

Harvest larvae using the Baermann
technique

Question 19

Increases the chances of recovering Strongyloides from samples

Answer 19

Harada Mori or Test Tube Culture Method

Question 20

In Harada-Mori, The tube is incubated at __ for up to _ days.

Answer 20

24 - 28 C; 10 days

Question 21

In Examination of Sputum,

__ is the best specimen

Answer 21

First morning specimen

Question 22

If the patient cannot expectorate, __ may be used

Answer 22

10% sodium chloride or hydrogen peroxide

Question 23

In Examination of Urine,

__ is the best specimen

Answer 23

First morning urine is the best specimen

Question 24

Examination of Urine,

May be used to detect _ and _

Answer 24

Trichomonas vaginalis and Schistosoma
haematobium

Question 25

Liver aspirates for the demonstration of __ in amebic liver abscess cases

Answer 25

Entamoeba histolytica trophozoites

Question 26

A capsulated yarn is swallowed by the patient and reach the duodenum

Answer 26

Intestinal intubation or Entero test/String test

Question 27

Retrieve after __ and examine the mucoidal material clinging to the yarn

Answer 27

4 hours

Question 28

EXAMINATION OF TISSUE ASPIRATES
* Cutaneous or skin aspirates
*Cutaneous ulcerations in ___
* Aspirate below the ulcer bed using a sterile needle
* Prepare a smear and stain with ___

Answer 28

leishmaniasis; Giemsa stain

Question 29

EXAMINATION OF
CSF
* May demonstrate the presence of the __ and the __
*Immediate examination is needed within __
* CSF must be centrifuged at __ discard the supernatant, and examine the sediment

Answer 29

trypomastigotes of
Trypanosoma spp. and trophozoites of Naegleria

20 minutes

7,000 g for 10 minutes,

Question 30

Useful in the diagnosis of Trichinella spiralis infection (presence of encapsulated
larvae)

Answer 30

Muscle Biopsy

Question 31

Muscle biopsy is Useful in the diagnosis of ___ (presence of encapsulated
larvae)

Answer 31

Trichinella spiralis infection

Question 32

Small pieces of muscles are pressed between _ glass slides

Answer 32

two

Question 33

Can reveal the presence of deposited Schistosoma japonicum eggs

Answer 33

Rectal Biopsy

Question 34

Rectal Biopsy Can reveal the presence of deposited __

Answer 34

Schistosoma japonicum eggs

Question 35

is a
routine method of stool examination
primarily useful in the detection of
motile protozoan trophozoite

Answer 35

Direct Fecal Smear

Question 36

This is done by sampling the perianal skin
using a strip of cellulose tape attached onto
a glass slide.

Answer 36

Cellulose tape or scotch tape method