Clearing

Question 1

Excessive clearing may cause

Answer 1

brittleness

Question 2

Makes tissue ___

Answer 2

Transucent

Question 3

Characteristics of good clearing agent

Answer 3

Should be miscible with alcohol
should be miscible with paraffin wax
should not produce tissue shrikage
should make tissue transparent
should not dissolve analine dyes

Question 4

Most common clearing agents

Answer 4

Xylene
Toulene
Benzene

Question 5

For urgent biopsies

Answer 5

Benzene

Question 6

Miscible in both alcohol and paraffin

Answer 6

Toulene

Question 7

TSE transparent

Answer 7

Xylene

Question 8

TSE transparent

Answer 8

Xylene

Question 9

Carcinogenic

Answer 9

Benzene

Question 10

Tough tissues and large speciments

Answer 10

Chloroform

Question 11

Miscible in both alcohol and paraffin

Answer 11

Toulene

Question 12

Toxic to liver

Answer 12

Chloroform

Question 13

For CNS, smooth muscle

Answer 13

Cedarwood oil

Question 14

Milky on prolonged storage

Answer 14

Cedarwood oil

Question 15

P: for insects, embryos and delicate tses

Answer 15

Aniline oil

Question 16

Mnimum shringkage

Answer 16

Clove oil

Question 17

Similar to chloroform but cheaper, dangerous to inhale on prolonged exposure

Answer 17

Carbon tetrachloride

Question 18

Non toxic but with offensive odor and should be used in well ventilated room

Answer 18

Tetrahydrofuran

Question 19

For double embedding technique

Answer 19

Methyl benzoate and methyl salicylate

Question 20

Used when clearing directly from water

Answer 20

G

Question 21

temperature for infiltration

Answer 21

52-60 degrees celcius

Question 22

Process that removes tge clearing agent

Answer 22

Impregnation

Question 23

fills the tissue cavities

Answer 23

Impregnation

Question 24

Permeates tissue w/ a support medium

Answer 24

Impregnation

Question 25

If impregnation is incomplete?

Answer 25

Airholes in tissue sections

Question 26

3 types of general tissue impregnation

Answer 26

1. Paraffin Wax Impregnation 2. Celloidin Impregnation 3. Gelatin Impregnation

Question 27

Requires ___ or more changes of paraffin wax

Answer 27

2 or more

Question 28

Melting point of paraffin wax

Answer 28

56-58 degrees C at room temperature

Question 29

Never overheat only ___degrees celcius

Answer 29

>60

Question 30

Paraffin oven must be maintained ___ above the MP of wax

Answer 30

2-5 C

Question 31

Rapid processing time of PW

Answer 31

24hrs

Question 32

Three ways of PW Impregnation

Answer 32

1. By manual processing 2. By automatic processing (Autotechnicon & Elliot bench-type) 3. By vacuum embedding

Question 33

Most common water soluble wax

Answer 33

Carbowax

Question 34

Methods of celloidin infiltration

Answer 34

1. Wet 2. Dry 3. Nitrocellulose

Question 35

arrangement of the tissue in a precise position in the mold during embeddong

Answer 35

Orientation