Development One (Test Two) Flashcards
How did early scientists answer the central question of “how does a single celled zygote become a fully functional organism”?
They thought of the idea of pre-formation.
Preformation meant that the being inside was already formed and they pictured it as a miniature adult inside the sperm or egg.
How did Kaspar Friederich Wolff demonstrate that there was no preformed chick in the early egg?
He noticed that the undifferentiated granular material became arranged into layers
The layers thickened, thinned, and folded to produce the embryo.
What did Kaspar Friederich Wolff call the process of forming an embryo?
He called it epigenesis (origin upon or after)
What did Kaspar Friederich Wolff not know?
How the fertilized egg, containing the building material, actually developed.
He didn’t know what directed the process.
How are current ideas of development similar and different to those of Wolff’s?
They are similar in that they are essentially epigenetic in concept.
They are different in that we know much more about what directs growth/development, hence the discovery of mitosis.
We now understand cellular diversity as a hierarchy of developmental decisions.
What is key to the definition of Fertilization?
The moment of fertilization is when the two gamete nuclei fuse.
What first happens when the sperm finds egg?
Firstly, the sperm must penetrate the jelly layer that surrounds the egg.
What happens secondly in the process of fertilization?
After penetration of the jelly layer, the sperm contacts the vitelline envelope
The vitelline envelope is a thin layer that is above the egg’s plasma membrane.
What makes sure the sperm is of the same species as the egg?
(Lots of different sperm floating around in the ocean!)
The enzymes on the vitelline envelope make sure the sperm is the same species because the sperm’s acrosomal process must have the correct enzymes that match the envelope in order to bind.
What is/are the yolk granules?
“Packages of nutritional value”
(inside the egg in the cytoplasm)
What is polyspermy, and how does it affect development?
Polyspermy is the entry of more than one sperm to an egg, and it is detrimental to normal development.
What is “fast block”?
First, quick, step in prevention of polyspermy
Electrical potential of the membrane changes when a sperm (slightly negative) enters because the inside of egg becomes positively charged, which spreads across the membrane and prevents entry of any other sperm.
What is “slow block”?
Second prevention of polyspermy after the fast block, cortical granules on the edge of the plasma membrane release contents between the membrane and the vitelline envelope once a sperm has fused with the plasma membrane.
This creates an osmotic gradient, which causes water to rush into the space and the envelope swells.
This swelling lifts away all the bound sperm, except the one that fused with the membrane.
It also modifies the eggs surface so that other sperm cannot enter.
What happens to the sperm after the sperm and egg membranes fuse?
The sperm loses its flagellum, and the nuclear envelope of the sperm breaks apart to allow the chromatin to expand (nucleus gets bigger)
Describe the process of pronuclei fusion.
The enlarged sperm nucleus (pro-nucleus) migrates inward to contact the female pro-nucleus, which creates a diploid zygote nucleus
What are three cytoplasmic changes does the new diploid zygote go through to prepare for cleavage?
- protein synthesis
- Constant DNA replication
- Cytoplasm repositions stuff to activate or repress certain genes
What is cleavage?
Cleavage is a series of uneven mitotic divisions.