Delayed Type Hypersensitivity Testing

Question 1

evaluates functionality of immune system

Answer 1

DTH

Question 2

Scale on Gel and Coombs scale

Answer 2

Type IV

Question 3

reduction or lack of an immune response to specific antigen

Answer 3

anergy

Question 4

when patient does not respond to any antigens

Answer 4

anergic

Question 5

procedure used to determine a persons immune response

Answer 5

anergy panel

Question 6

Indications for DTH

Answer 6

evaluate immunity of patients with possible immune deficiency

assess immuno-competency of malnourished patients

assess patients for possible chemotherapy

HIV staging

Pre and post surgery patients to assess risks

Question 7

1st strength for PPD and Candida

Answer 7

PPD - 5 Tuberculin Units (TU)

Candida - 20 Protein Nitrogen Unit (PNU)

Question 8

2nd Strength for PPD and Candida

Answer 8

PPD - 5 TU

Candida - 200 PNU

Question 9

Time Interval DTH

Answer 9

Reaction start at 24 hours
Peak at 48 hours
Subside at 72 hours

Question 10

Dose of all DTH

Answer 10

0.1 mL

Question 11

Patient Screening

Answer 11

Past + PPD
Hx of BCG
Return 48-72 hours
Taking any steroids or receiving radiation therapy?
Live vaccine past 28 days

Question 12

False Negative Results

Answer 12

too old/young
no prior exposure
active viral infection
solutions are weak or outdated
improper placement
recent live virus

Question 13

False Positive

Answer 13

rubbing/scratching/ agitating
lotions/soaps/oils
cologne/perfumes
wrong diluent used when preparing antigen