Decoding Information in DNA---> end of The Origins of Life Flashcards
What functions do proteins have?
Enzyme and structural purpose, determining our traits
Are proteins built directly from DNA?
No.
What is RNA?
Ribonucleic acid, a nucleic acid made of nucleotides.
What is the structure of RNA?
Single helix
In how many ways does RNA differ from DNA?
Three.
Which five carbon sugar do RNA nucleotides contain?
Ribose (instead of deoxyribose like DNA.)
What is the difference between deoxyribose and ribose?
Ribose contains one more oxygen then deoxyribose.
What is the difference in nitrogenous bases between DNA and RNA?
They both contain adenine, guanine, and cytosine, but instead of thymine like DNA, RNA contains uracil, complimentary to adenine.
Transcription
The process by which a gene’s instructions for making proteins are transferred to an RNA molecule.
Where does transcription occur?
The nucleus, where the DNA is located.
RNA polymerase
An enzyme that binds complementary RNA nucleotides during transcription.
What is the first step of transcription?
RNA polymerase binds to a special start sequence of DNA.
In transcription, what does the RNA polymerase do after binding to the special start sequence of DNA?
It unwinds and separates the two strands of the double helix.
In transcription, what happens after the RNA polymerase unwinds and separates the double helix?
It adds and links complementary RNA nucleotides as it reads the DNA.
What base pairing rules of DNA does transcription follow?
All of them except that in RNA uracil pairs w adenine instead of thymine pairing w adenine.
What does transcription proceed until?
RNA polymerase reaches a stop sequence of bases that marks the end of a gene.
What happens after transcription is completed?
The two strands of DNA close up, reforming the double helix and the RNA strand separates from the DNA.
What are the practical differences between transcription of RNA and DNA replication?
-One strand of RNA is produced vs. two strands of DNA-In DNA replication, both strands of DNA are used as templates vs. in transcription only one strand is used
What can be formed by transcription?
Different types of RNA.
What is mRNA?
Messenger RNA, which carries the instructions for building a protein from the DNA gene to the ribosome.
What happens w mRNA at the ribosomes?
The instructions are “read” which code for specific amino acids
What do many amino acids joined together make?
A protein
Codons
Series of three nitrogenous bases on the mRNA
What are mRNA instructions written as?
Codons
Genetic code
Shows all 64 possible codons and the amino acids they code for.
Translation
Using RNA to read the instructions on mRNA and put together the amino acids that make up the protein.
Where does translation take place?
The cytoplasm, at the ribosomes.
How many other types of RNA help the process of translation? What are they?
2, tRNA and rRNA.
What is tRNA?
Transfer RNA.
What is transfer RNA?
Folded molecules that carry a specific amino acid on one end and has an anticodon on the other.
Anticodon
Three base sequence that is complimentary to an mRNA codon.
What is rRNA?
Ribosomal RNA.
What is ribosomal RNA?
It helps make up the ribosomes.
Where does translation occur?
The ribosomes.
First step of translation
mRNA leaves nucleus, enters cytoplasm and attaches to a ribosome
What happens when mRNA attaches to a ribosome in translation?
The ribosome reads the codon on the mRNA and a tRNA molecule w the complementary anticodon arrives and binds to the codon.
What happens in translation when the tRNA w the right anticodon binds to the codon?
The tRNA then separates from the amino acid and leaves the ribosome.
What happens when the tRNA leaves the ribosome?
The ribosome moves along to the next codon and a new tRNA molecule takes its place on the mRNA strand.
What happens to the new amino acid brought in in translation?
Joins the others with a peptide bond
Polypeptide chain
chain of amino acids
What happens at the end of translation when the new amino acid binds to the others?
A polypeptide chain grows until the ribosome reaches a stop codon, and the chain lets go, folding into a protein molecule
Mutation
Change in DNA of a gene
How are mutations passed to offspring?
Thru gametes (sex cells) of affected individual
What happens when a mutation occurs in the somatic cells of an individual?
They won’t affect the offspring of that individual
Gene rearrangments
Mutations that move an entire gene to a new location
Translocation
Movement of part of a chromosome to a different location
Where can translocations happen?
Within the same chromosome or between different ones
Gene alteration
Mutation that changes a gene itself
Substitution mutation
Nucleotide w different nitrogenous base replaces original nucleotide
What may result from a substitution mutation?
Missense mutation or silent mutation
Missense mutation
Results in change in single amino acid in final polypeptide
Silent mutation
New codon codes for same amino acid; no change occurs
Insertion
Piece of DNA inserted into gene
Deletion
Segment of gene is lost
What can insertion and deletion affect?
Change the order of bases in codons, resulting in totally different codons