Deck 6 Flashcards
PCR test is used for detection of:
Genome or part of genome
Staphylococci are gram:
positive
Division of cell cultures according to the growth potential:
Primary and established
Phylogenetic tree shows:
In graphic form, developmental relationship between studied strains
Which media is used for cultivation of salmonella?
MacConkey agar
Which method is used for microscopic detection of funghi?
Native with KOH
What are advantages of real-time PCR compared to classical PCR?
Faster results and easy
Give an example of non-contagious infectious disease:
Blue-tongue
Describe risk assesment in epizootiology:
Pathogenicity, mode of transmission, hosts, zoonotic or not, mortality
What does the approach one health mean?
Human health and animal health are interdependent and bound to the health of the ecosystem in which they excist
Source of infection is:
Macroorganisms, in which etiological agent survives, multiplies and is excreted
Complement fixation test is used for detection of:
antibody
In sandwich ELISA positive result is:
Change of colour
What is tropisms of pathogens?
Monotropic, pantropic, polytropic
Name at least 3 tests for cellular immunity evaluation:
Flow cytometry
Complete blood count
Lymphocyte proliferation assay
What is the role of colostral immunity?
To provide passive immunity and protection against septicaemia and organ infections as well as protection of intestine and GIT bacteria multiplication
Name clinical signs indicating failure of passive transfer of immunity:
Profuse diarrhoea
Dehydration
Respiratory or systemic infection
Give an example of facultative pathogens:
Candida
Acanthomoeba
What is epizootiological triad?
Relationship between host/animal population, etiological agent and environment
Immunofluorescence detects:
Antigen
Boya’s method is for detection of:
Mycobacterium
Haemagglutination detects:
Virus (Ab)
Blocking ELISA is used for detection of:
Antibody
Annealing temperature of PCR:
45-60*C
Skin contamination is cleaned by:
0.5% chloramine B
4 stages of phagocytosis:
Chemotaxis
Adherence
Ingestion
Digestion
COnjugation involves:
Ig, serum and fluorochrome
What is the principle of immunofluorescence?
To use marked antibodies of immune serum against antigen which we want to vizualise
Define indirect transmission:
Passing of infectious agents between inanimate or animate objects
Define a panzootic process:
Disease occurring over a wide area and subsequently affecting a large proportion of the population
