Deck 1

Question 1

What is the principle of PCR?

Answer 1

DNA replication

Question 2

What is the causative agent of Rift valley fever?

Answer 2

Bunyavirus phlebovirus

Question 3

What are susceptible hosts of foot and mouth disease?

Answer 3

Cloven-hoofed animals

Question 4

What causes ringworm in cattle?

Answer 4

Trichopyton verrusocum

Question 5

What is the one health concept?

Answer 5

It is a collaberative, multisectorial and transdiciplinary approach, working at local, regional, national and global levels of achieving optimal health outcomes recognizing the interconnection between people, animals, plants and their shared environment.

Question 6

What are the stages of infectious process?

Answer 6

Incubation
Prodromal
Manifestation
Final

Question 7

Transmission of borreliosis is by the:

Answer 7

Ixodes tick

Question 8

Causative agent of classical swine fever is:

Answer 8

Pestivirus

Question 9

Causative agent of aleutian mink parvovirus:

Answer 9

Amdovirus parvovirus

Question 10

What causes enterotoxaemia in poultry?

Answer 10

Clostridia perfringens

Question 11

What is a calf born with?

Answer 11

Agammaglobulinemia

Question 12

How does bacterial culture grow on agar?

Answer 12

Monolayer, to the edges

Question 13

MAT is used to diagnose which bacteria?

Answer 13

Leptospirosa

Question 14

Biosafety level 4 are used in which disease?

Answer 14

Ebola, small pox

Question 15

Inhibition haemagglutination test are positive when?

Answer 15

No agglutination, button

Question 16

Natural foci are described by:

Answer 16

Geographically and seasonally

Question 17

Swine strepto-meningitis is:

Answer 17

Zoonotic

Question 18

Tetanus is (oxygen):

Answer 18

anaerobic

Question 19

What is the main principle of ELISA:

Answer 19

Combining Ag + Ab visualised by an enzyme and chromogen using spectrophotometer

Question 20

Name enzymes often used in ELISA:

Answer 20

Horseradish peroxidase, beta galactosidase, alkaline phosphatase, lysozyme

Question 21

Indirect ELISA is used for:

Answer 21

Looking for antibody in serum: plates are incubated with antigen, serum is added and antibody binds, then serum is washed, and antiglobulin linked to enzyme is then added and binds to antibody –> colour reaction.

Question 22

Sandwich ELISA is used for:

Answer 22

Looking for antigen coat well with specific antibody: antigen solution is added and bound by captured Ab, washed and specific Ab-enzyme labelled antiglobulin is added –> colour reaction

Question 23

Competitive ELISA is used for:

Answer 23

Known amount of labelled Ag competes with investigated amount of non-labelled Ag for Ab binding sites on surface

Question 24

Blocking ELISA is used for:

Answer 24

Purified Ag binds to solid phase, test serum is added, incubated and washed. Labelled Ab against Ag is added. Negative colour reaction = presence of Ab, positive = no Ab

Question 25

Stain used for mycobacterium:

Answer 25

Ziehl-Neelsen

Question 26

Principle of virus neutralization is:

Answer 26

Neutralization of virus by specific antibodies in the serum tested:
- cytopathic effect of the virus in a cell culture is blocked
- in tubes or microtitre plates
- positive serum contains specific antibodies that neutralize virus and virus cannot damage cells

Question 27

What are PCR components?

Answer 27

Template DNA
Nucleotides
Primers
Buffers
DNA polymerase
Thermocycler

Question 28

Rabies way of transmission:

Answer 28

Primarily through saliva, bite wounds or infected saliva entering an open cut or wound or mucus membrane.
Sources of virus is saliva and brain tissue.
Occasionally by infected aerosol or oral.

Question 29

Listeriosis way of transmission:

Answer 29

Alimentary

Question 30

What is OIE?

Answer 30

Office International de Epizootes (World Organisation for Animal Health)