Deck 1 Flashcards
What is the principle of PCR?
DNA replication
What is the causative agent of Rift valley fever?
Bunyavirus phlebovirus
What are susceptible hosts of foot and mouth disease?
Cloven-hoofed animals
What causes ringworm in cattle?
Trichopyton verrusocum
What is the one health concept?
It is a collaberative, multisectorial and transdiciplinary approach, working at local, regional, national and global levels of achieving optimal health outcomes recognizing the interconnection between people, animals, plants and their shared environment.
What are the stages of infectious process?
Incubation
Prodromal
Manifestation
Final
Transmission of borreliosis is by the:
Ixodes tick
Causative agent of classical swine fever is:
Pestivirus
Causative agent of aleutian mink parvovirus:
Amdovirus parvovirus
What causes enterotoxaemia in poultry?
Clostridia perfringens
What is a calf born with?
Agammaglobulinemia
How does bacterial culture grow on agar?
Monolayer, to the edges
MAT is used to diagnose which bacteria?
Leptospirosa
Biosafety level 4 are used in which disease?
Ebola, small pox
Inhibition haemagglutination test are positive when?
No agglutination, button
Natural foci are described by:
Geographically and seasonally
Swine strepto-meningitis is:
Zoonotic
Tetanus is (oxygen):
anaerobic
What is the main principle of ELISA:
Combining Ag + Ab visualised by an enzyme and chromogen using spectrophotometer
Name enzymes often used in ELISA:
Horseradish peroxidase, beta galactosidase, alkaline phosphatase, lysozyme
Indirect ELISA is used for:
Looking for antibody in serum: plates are incubated with antigen, serum is added and antibody binds, then serum is washed, and antiglobulin linked to enzyme is then added and binds to antibody –> colour reaction.
Sandwich ELISA is used for:
Looking for antigen coat well with specific antibody: antigen solution is added and bound by captured Ab, washed and specific Ab-enzyme labelled antiglobulin is added –> colour reaction
Competitive ELISA is used for:
Known amount of labelled Ag competes with investigated amount of non-labelled Ag for Ab binding sites on surface
Blocking ELISA is used for:
Purified Ag binds to solid phase, test serum is added, incubated and washed. Labelled Ab against Ag is added. Negative colour reaction = presence of Ab, positive = no Ab
Stain used for mycobacterium:
Ziehl-Neelsen
Principle of virus neutralization is:
Neutralization of virus by specific antibodies in the serum tested:
- cytopathic effect of the virus in a cell culture is blocked
- in tubes or microtitre plates
- positive serum contains specific antibodies that neutralize virus and virus cannot damage cells
What are PCR components?
Template DNA
Nucleotides
Primers
Buffers
DNA polymerase
Thermocycler
Rabies way of transmission:
Primarily through saliva, bite wounds or infected saliva entering an open cut or wound or mucus membrane.
Sources of virus is saliva and brain tissue.
Occasionally by infected aerosol or oral.
Listeriosis way of transmission:
Alimentary
What is OIE?
Office International de Epizootes (World Organisation for Animal Health)
