Deck 5 Flashcards
Division of media used for cell culture
Liquid and solid
In blocking ELISA positive result is:
no colour
Give an example of facultative pathogens:
Bacteria: Salmonella, Staphylococcus spp., Listeria spp., Pseudomonas aeruginosa
Animal notifiable diseases are reported to:
World organisation for animal health
What are the stages of epizootic process?
Interepizootic
Preepizootic
Ascending
Culmination
Descending
Postepizootic
What is epizootiological triad?
Animal population, etiological agents and environmental factors
What is an abortive infection?
Infection that disappears after prodromal period
Who can enter biosafety 3 laboratories?
Authorized staff only, and their stay is controlled and restricted to necessary time
List 3 methods alternative to use of living laboratory animals:
Chorioallantoic membrane (bird embryos and associated tissues)
Cell cultures
Tissue cultures
Organoids
Yeasts
Computer simulations
What are disadvantages of the chorioallantoic membrane method?
Physiology of mammals can be different to that of birds, some avian reagents are hard to obtain, vasculogenesis
What does confluence describe in the context of cell cultures?
Proliferation under the appropriate conditions until they occupy all the available substrate
Sabourad’s glucose agar is used for cultivation of:
Yeasts
PCR methods are used for:
Direct detection of pathogen
Cytopathic effect is:
Destruction of the cells after virus inoculation
What are the main reagents in ELISA?
Antigen
Antibody
Enzyme
Chromogen