Day2 Flashcards

1
Q

What spp. is described?

  • frequent lab contaminants
  • widely distributed, particularlysoil
  • large, GPR , square ended spore forming
  • Colonies are spreading, dry, granular, gray-green and usually beta
A

Non pathogenic Bacillus spp.

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2
Q

Bacillus spp that can cause food poisoning but usually non pathogenic

A

Bacillus cereus

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3
Q

Bacillus spp that is usually a contaminant (non pathogenic)

A

Bacillus subtilis

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4
Q

What organism is the primary human pathogen in the Bacillus genus ?

A

Bacillus anthracis

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5
Q

Why is the military concerned about B.anthracis?

A

It has a potential to be used as a biological weapon

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6
Q

Describe the appearance of B.anthracis spores

A

Ellipsoidal to cylindrical centrally located spore

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7
Q

What organism is described: frequently long chains, bamboo or “box car” appearance.

A

Bacillus anthracis

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8
Q

Why is motility and hemolysis particularly important when differentiating between Bacillus spp.

A
  • Bacillus anthracis is non-motile; other species are motile

- Bacillus anthracis is gamma hemolytic; other species are usually beta

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9
Q

What gives bacillus anthracis its virulence?

A

Encapsulated and toxin-producing

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10
Q

Describe Pulmonary Anthrax.

A

Woolsorter’s disease, spores INHALED during shearing, sorting or handling of animal hair or skin

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11
Q

Describe Gastrointestinal Anthrax

A

Most rare; bacilli or spores gain entrance into the body when swallowed or ingested

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12
Q

Describe Cutaneous Anthrax

A

Infection initiated by entrance of bacilli through abrasions in the skin

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13
Q

What extreme caution must be taken when handling Bacillus anthracis?

A

Using Bacteriological safety hood

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14
Q

Another name for Corynebacterium spp?

A

Diptheroids

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15
Q

What organism is described:

  • gram positive slender pleomorphic rods
  • colonies are ORANGE to CREAM in color
  • GARLIC odor
  • Non pathogenic; NF of skin and mucous membranes
A

Corynebacterium spp.( Diptheroids)

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16
Q

Describe Corynebacterium diphtheriae.

A
  • small Gram positive
  • very pleomorphic, slender bacilli
  • found in CHINESE LETTER or PALISADES configuration
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17
Q

What are the 3 subspecies of Corynebacterium diphtheriae?

A

Gravis
Intermedius
Mitis

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18
Q

What do the three subspecies of Corynebacterium diphtheriae all have in common?

A

They all cause “Diphtheria”

19
Q

What are the three types of media used for Corynebacterium diphtheriae?

A

BAP
Loeffler’s Serum
Cystine-Tellurite

20
Q

Purpose of Loeffler’s Serum Media?

A

promotes the pleomorphic appearance; enhances identification of meta chromatic granules

21
Q

Purpose of Cystine-Tellurite?

A

Allows for colony morphological differentiation of subspecies

22
Q

Describe colony of C.diphtheriae mitis.

A
small
black
shiny
convex
round
beta hemolytic
23
Q

Describe colony for C.diphtheriae intermedius.

A

Small
Flat
Non hemolytic

24
Q

Describe colony of C.diphtheriae gravis.

A

Large
Dark grey center
Brittle
Non hemolytic

25
Q

What are the symptoms of diphtheria? What causes them?

A

Soreness in throat, pharynx and larynx; followed by myocarditis and neuritis caused by and EXOTOXIN

26
Q

True or false:

Corynebacterium spp. are motile

A

False, they are non motile

27
Q

What is Elek?

A

Toxin testing used to differentiate pathogenic Corynebacterium diphtheriae from non pathogenic

28
Q

What is used to ID Corynebacterium diphtheriae?

A

Toxin testing(Elek) and Carbohydrate testing

29
Q

What organism is a major pathogen of the Listeria spp. and is most often encountered in clinical lab?

A

Listeria monocytogenes

30
Q

What diseases does Listeria monocytogenes cause?

A
  • Nonspecific febrile illness in pregnant women, which may lead to stillbirths
  • Meningitis in adults
  • Sepsis and/or meningitis in neonates
31
Q

Describe motility of Listeria spp.

A

25 degrees Celsius= umbrella motility

37 degrees Celsius= non-motile

32
Q

What are the characteristics of Lactobacillus spp?

A

Catalase neg
Non-motile
Non-spore forming
Facultative anaerobes or microaerophilic

33
Q

True or false:

Lactobacillus is Normal flora of the oral, intestinal and vaginal tract.

A

True

34
Q

What disease does Lactobacillus cause?

A
  • Usually non pathogenic
  • isolated in cases of endocarditis, septicemia and meningitis
  • Frequent contaminant in urine cultures
35
Q

Describe hemolysis characteristics of Listeria spp.

A

Narrow zone of beta hemolysis

36
Q

What is characteristic about the morphology/ gram stain of Gardnerella vaginalis?

A

resembles a gram negative bacilli but has the cell wall of a gram positive organism; appears GRAM VARIABLE

37
Q

Why is Gardnerella vaginalis considered to be marker of bacterial vaginosis rather than a causative agent?

A

Gardnerella vaginalis can be part of many women’s normal flora.

38
Q

What is a clue cell?

A

Epithelial cells covered by gram variable bacilli

39
Q

What organism considered to be a veterinary pathogen ?

A

Erysipelothrix rhusiopathiae

40
Q

What organism causes a cellulitis-like lesion on the hands?

A

Erysipelothrix rhusiopathiae

41
Q

What organism cannot be clinically distinguished from BSGA?

A

Arcanobacterium haemolyticus

42
Q

What organism produces toxins: Phospholipase D and Hemolysin, and the enzyme Neuramindase?

A

Arcanobacterium haemolyticus

43
Q

What organism was formerly classified in the genus Corynebacterium?

A

Arcanobacterium haemolyticus