Day 8 Flashcards
Slow growers ; require several weeks to see visible colonies; high concentrations of loped in cell wall
Acid fast Bacilli
What does acid fast bacilli require to grow?
Increased CO2 and 37degrees Celsius
What is the microscopic appearance of acid fast bacilli?
Slightly curved or straight bacilli; may see mycelia
What are the 4 classifications for AFB?
Runyon Group 1 Photochromogen
Runyon Group 2 Scotochromogen
Runyon Group 3
Runyon Group 4
which Runyon Group is described?
Produce pigment after exposure to light
Group 1
which Runyon Group is described?
Produce pigment in the dark and light.
Group 2
which Runyon Group is described?
Slow growing; nonpigmented
Group 3
which Runyon Group is described?
Rapid growers
Group 4
What is the etiological agent of tuberculosis?
Mycobacterium tuberculosis
Causes human and animal tuberculosis; acquired through unpasteurized milk and undercooked beef
Mycobacterium bovis
Causative agent of Hansen’s disease(leprosy) ; causes skin lesions
Mycobacterium leprae
Most common mycobacterium found in AIDS patients in US
Mycobacterium avium-intracellulare
What is the most widely used specimen for mycobacterium?
Sputum
For decontamination and digestion, what are the 3 chemicals used?
Sodium hydroxide (2-4%)(mostcommon) Zephaniah-trisodium-phosphate(13%) Permits better recovery of organisms N-acetyl-L-cysteine Best for digestion of mucoprotein
What media is used for AFB?
Egg and potato based
Contains machalite green inhibitory dye
Lowenstein Jensen
Contains a higher concentration of machalite green that LJ
Pertragnani medium
Detects early growth of microscopic colonies; used for antimicrobial sensitivity testing
Middlebrook 7H10
Name a partially acid fast bacilli
Nocardia spp.
Most common sexually transmitted bacterial agent; associated with: Trachoma Trachoma Inclusion Conjunctivitis(TIC) Lymphogranuloma venereum(LGV) Genital tract disease
Chlamydia spp.
Chlamydia species that mainly affects birds
Chlamydia psittaci
Chlamydia species that is isolated in patients with respiratory infections
Chlamydia pneumoniae
How do you collect Chlamydia specimens?
By vigorously scraping the involved epithelial cells; cervical and urethral scrapings
What spp. requires embryonated chicken egg cell cultures
Chlamydia
What is the most common method of detection of Chlamydia?
ELISA and immunofloresence
What class does Mycoplasma belong too?
Mollicutes
“Fried egg” appearance; Supravital dyes (Dienes method) dark blue center and light blue edges
Mycoplasma spp.
What organism is asymptomatic And known as “walking pneumonia “?
Mycoplasma pneumoniae
Causes spontaneous abortions and post-partum fever and respiratory diseases in neonates
Mycoplasma hominis
What does mycoplasma spp. Need to grow
95% nitrogen and 5%CO2
