Day 6 Flashcards
An ultrafiltrate of blood that fills the spaces within and around the central nervous system
Cerebrospinal fluid
3 connective tissues that make up the meninges
Dura mater
Arachnoid
Pin mates
Where is CSF formed
Found in plexuses which are thin walled capillaries
CSF has more ______,_______,_______ but less _______ than plasma
Sodium hydrogen & chloride
Protein
How much CSF Is formed daily?
500 ml every 8 hours
3 major functions of CSF
Supply nutrients to the nervous tissue
Remove metabolic waste
Cushion the brain and spinal cord against trauma
Where on the spinal column is CSF collected from?
In between the 3rd & 4th or 4th & 5th vertebrae
Where does each CSF tube tested
Tube 1: chemical and serology testing
Tube 2: microbiology
Tube 3: hematology
What is the most frequently performed chemical test on CSF
CSF protein
Csf protein blues are higher in what indivi
Infants and older folk
What is the most common cause of elevated CSF protein
Meningitis & hemorrhage
What test is performed to fraction CSF protein
Electrophoresis
The recommended support medium for electrophoresis is ______ followed by staining with _______
Agarose gel
Coomaise blue
Characteristics oligolonal bands observed in the gamma region of a CSF protein electrophoresis indicates what disease?
Multiple sclerosis
Norma CSF is what percent of the plasma glucose level
60-80%
When should a CSF for glucose be collected
2 hours before collecting CSF
Why should samples for glucose CSF be collected immediately
Glycolysis occurs rapidly in CSF
5 microbiology test that give preliminary diagnosis
Gram stain AFB stain India ink stain Counter immunoelectrophoresis Latex agglutination
A CSF sample for gram stain analysis should be centrifuges at
1500 RPM at 15 minutes
What is the most common cause of false positive reactions on CSF late text agglutination test
Interference of rheumatoid factor‘s
How soon should a CSF cell count occur after collection ?why?
30 minutes to prevent RBC and WBC from Lysing
When is CSF WBC morphology performed
Counts > 30wbcs/uL
A high CSF WBC with a high percentage neutrophils neutrophils is indicative of what disease
Bacterial meningitis
An elevated CSF WBC count with a high percentage of lymphocytes and monocytes suggest what disease
Meningitis from viral fungal or parasitic faction
Serological CSS examination is typically associated with diagnosis of what disease
Tertiary or neurosyphilis
Treatment with_________ In early stages of syphilis has greatly reduce the number of neurosyphilis cases
Penicillin
This fluid is a viscous fluid found in joint cavities
Synovial fluid
Synovial membrane secrete Mucopolysaccharide containing what
hyaluronic acid and a small amount of protein
A needle aspiration to collect synovial fluid is referred to as
arthro-centesis
List synovial fluid tube type for each area to be tested
Microbiology sterile heparinized
Hematology EDTA
Chemistry/ serology no anticoagulated tube
Inflammatory immuno logical problems associated with synovial fluid include
Rheumatoid arthritis and lupus
Why shouldn’t acetic acid be use for cell counts
It will cause and using clotting and said hypotonic Saline should be used to containing methylene blue
What is the two primary crystals found in synovial fluid and the disease is associated with them
Monosodium urate (Uric acid) Gout Calcium pyrophosphate Dihydrate CPPD Psyedogout
Three common chemistry test performed on synovial fluid
Glucose total protein uric acid
A needle aspiration to collect pleural fluid is referred to as
Thoracentesis
A needle aspiration to collect pericardial fluid is referred to as
Pericardiocentesis
I need a laceration to collect peritoneal fluid is referred to as
Paracentesis
Explain the difference between serous fluid transudate and exudate
Translates are caused by a mechanical error
Exudates Are caused by inflammatory process and may indicate infection or malignancy
Cell counts on serous fluid are done utilizing a
Electronics cell counter
What causes the possible milky appearance of plural fluid
Presence of Chylous material and pseudo-Chylous material
What chemistry test can differentiate between serous fluid exudates and transudates
Protein
Protein <3g/dL transudates
>3g/dL exudates
