Day 4 Flashcards
When must urine quality controls he performed? How many?
Each day patient testing occurs & when new reagent bottle is opened
Urine pH Is a reflection of what major function
Regulation of acid base content
What are pH indicators in the urine reagent strip? What causes each to react?
Methyl red- pH range of 4.4-6.2
Bromethyl blue- pH range of 6.0-7.6
What is the most indicative routine chemical test for kidney disease if detected early in the disease?
Urine protein
What is the primary protein found in urine
Albumin
What type of protein is detected in patients with multiple myeloma?
Serum (pre renal protein)
Explain one major cause of proteinuria
Abnormal amount of protein in your urine
What urine test us used to screen patients with diabetes in order to prevent damage to the kidneys
Microalbumin strip
What is the confirmatory test for protein
Sulfosalcylic acid SSA
What protein is missed on the reagent strip that can be detected on the confirmatory test?
Tubular proteins & Bence Jones protein
Glycosuria is commonly associated with what disease
Gestational diabetes
What is the back up/ confirmatory test for glucose?
Cliniest
Explain why a negative glucose dipstick but a positive back up/ confirmatory test is significant?
Clink test are not as sensitive as a dipstick so it could indicate an error w/ the dipstick
What does the presence of ketones in the urine indicate about the patient?
They maybe starving vomiting or weight reduction
What is the principle behind the urine reagent strip method for ketones
Acetoacetic acid reacts w/sodium’s nitroprusside to form a purple color
What is the back up test for ketones
Acetest
What tests(4) report out results like the following: “neg, trace, 1+,2+,3+,4+”
Protein
Glucose
Ketones
Bilirubin
What tests(2) report out results like the following: “neg, trace, 1+,2+,3+”
Blood
LE
What test reports out results like the following:
“1,4,8,12mg/dl”
Urobilinogen
What test reports out results like the following:
“neg or pos “
Nitrite
______ ___________ bacteria reduce nitrate to nitrite
Gram negative
________ at acid pH reacts with aromatic amine to form a ________ compound which reacts with 3-hydroxy-1,2,3,4-tetrahydobenz-(h)-quinolin to produce a pink color
Nitrite ; diazonium
Nitrite is detected by the _____ reaction
Griess reaction
What could a positive LE with no WBCs or bacteria in microscopic analysis be caused by?
Chlamydia spp.
Trichomonas spp.
In LE , why is diazonium salt added to the reaction pad?
Reduces reaction time to 2 minutes
What is another name for p-dimethylaminobenzaldehyde?
Erlich’s Reagent
What reagent is utilized in Multistix?
Erlich’s reagent
What methodology does Chemstrips use?
Diazo reaction where diazonium salt reacts with Urobilinogen in an ACID medium to produce a red color.
What is the principle for the Ictotest?
Based on Diazo Reaction; much more sensitive that reagent strip
What are 5 causes of hemoglobinuria?
Hemolytic anemia Transfusion reactions Severe Burns Infections Strenuous exercise
An oxygen binding protein found in cardiac and muscle tissue that reacts POSITIVELY with chemical test for blood
Myoglobin
What is the enzyme in the urine reagent strip that test for blood
Hemoglobin
What is the confirmatory test for bilirubin?
Ictotest
What is the methodology behind the urine reagent strip test for bilirubin
Bilirubin combines with color changing chemical
What reagent recuse with urobilingen on the urine reagent strip
Ehrlichs reagent
Why is it possible to have positive leukocyte esterase result but not see WBCs in the microscope?
Chalymadia or T. Vag
What is the methodology behind the urine reagent strip test for leukocyte esterase?
Diazo reactions