Danio and Arabidopsis Flashcards
D. Fish
Scientific name _____
Also known as ____
_____ used it first because it was easier to use over the mouse to manipulate genetically
Danio rerio
Zebrafish
George Streisinger
D. Zebrafish (Danio rerio)
Advantages:
They’re small, robust and cheaper than mice
They reproduce ____ giving a steady supply
They develop incredibly fast, with one day of development being 1 month of human embryo
Their embryos are transparent which allows easy observation.
They share _____ percent of genes. However ____ percent of genes associated w disease has a zebrafish counterpart
They have a unique ability to repair ____.
weekly
70 %
84%
Heart Muscle
D. Zebrafish (Danio rerio)
Applications:
Zebrafish is typically used in _____
However, they’ve also been used to study ____ and _____
They can also be used as a model for cancers, with being exposed to carcinogens leading to the development of benign and maligmant tumors in _____
They are used as ____
developmental biology
Angiogenesis and regeneration
teleosts
Whole-animal assays
D. Zebrafish (Danio rerio)
Limitations:
They need to live in an aquatic environment which can be expensive.
Cannot create specific gene Knockouts, mutations tend to be random
Its last common ancestor was _____ thus far remote from humans compared to ____ with rats
They’re also ____ which is not identical to humans when it comes to environmental temperature regulation
The genome also has multiple duplications as many genes have two copies
_____ intereferes with drug permeability
445 million years ago
96 million years ago
ectothermic
48 hpf chorion
E. Plant
Scientific name ______
Also known as ______
It is first described by physician ______
But _____ was the first researcher to publish an influential cytogenetic study
Arabidopsis thaliana
Rosette plant
Johannes Thal
Friedrich Laibach
E. Plant - Arabidopsis thaliana
Grows fast with as few as ____ to maturity. Seedlings can be grown in a petri dish, usually hundreds of them
It is typically used in _____ and ____
_____ a key component of _____ throigh which miRNAs act was discovered thanks ot leaf deformities
6 weeks
Biochemistry and plant development
ARGONAUTE
RNA-Induces Silencing COmplex (RISC)
E. Plant - Arabidopsis thaliana
Limitations:
Not all genes used by other plants can be represented in Arabidopsis
It’s small so it might be difficult to extract sparse _____with some things being absent from arabidopsis entirely
Some cell structures differ in arabidopsis
biochemicals
