Culturing Flashcards
3 types of culture media
- Agar plates
- Agar/slant tubes
- Broth “liquid” tubes
General all purpose media that supports the growth of most bacteria
Used in high volume labs
Ex: Nutrient agar, Trypticase soy agar
Basic Nutrient Media
A basic nutrient media that is good for growth of less fastidious (picky) bacteria
Nutrient agar
A basic nutrient media that will support the growth of most clinical bacteria
Trypticase soy agar
Type of media that is basic nutrient media with extra nutrients that help grow fastidiouc (picky) bacteria
Enriched media
An enriched media that is very common and contains 5% sheep blood (blood cells in sheep blood are broken down quickly) in trypticase soy agar
Supports the growth of most pathogens
Blood agar plate (BAP)
An enriched media which blood is added to and then heated and used to grow organisms that cause STDs (Ex: Brucellosis)
Chocolate agar
Type of media that contains some type of substance that selects for one type of bacteria and inhibits other bacteria from growing
Selective media
A type of selective media that selects for Gram + bacteria
Phenylethel alcohol agar (PEA)
Very popular selective media that selects for GNROD only
Good to use with a lot of different bacteria
Macconkey agar (MAC)
Type of media that selects for a group of bacteria AND contains an indicator (visible change by the metabolic activity of the bacteria)
Differential media
Type of differential media that colonies change color if bacteria FERMENT LACTOSE
Pink = lactose fermentor
Clear = non-lactose fermentor
Macconkey (MAC)
Type of differential media that selects for Gram - enteric pathogens and inhibits all normal flora
Good for salmonella and shigella
Checks for sugar fermentors = salmon colored
Non sugar fermentors = blue/green
Hektoen-Enteric agar
A differential media that is used to classify bacteria according to their ability to ferment
Yellow = indicates fermentation
Red = no fermentation
Triple Sugar Iron (TSI)
A differential media that selects for genus Staphylococci and can be used to isolate Staph aureus
Yellow = organism can ferment mannitol
Red = non-pathogenic staph
Mannitol Salt Agar
Media type that is usually a broth and selects for growth of particular organisms and contain extra nutrients to allow bacteria to grow better
Used for fecal cultures
Selective broth media
Broth that selects for and enriches enteric pathogens and inhibit normal bowel flora
Selenite
Broth that suppresses coliform (gut) organisms
Tetrathionate broth
Misc media that is a useful, all purpose broth and used to increase the number of organisms before they are plated onto a solid medium
Brain-heart infusion
Misc broth media that determines motility of bacteria
Motility media
Misc media that tests for detection of a bacteria’s ability to produce indole
Indole test media
Creates partial hemolysis of blood agar
Narrow band of green, slimey discoloration around bacterial growth
Alpha
Creates complete hemolysis of blood agar
Clear band around bacterial growth
Beta
Created by non-hemolytic bacteria
No change of blood agar
Gamma
