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Clin Lab IV > Culturing > Flashcards

Culturing Flashcards

1
Q

3 types of culture media

A
  1. Agar plates
  2. Agar/slant tubes
  3. Broth “liquid” tubes
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2
Q

General all purpose media that supports the growth of most bacteria
Used in high volume labs
Ex: Nutrient agar, Trypticase soy agar

A

Basic Nutrient Media

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3
Q

A basic nutrient media that is good for growth of less fastidious (picky) bacteria

A

Nutrient agar

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4
Q

A basic nutrient media that will support the growth of most clinical bacteria

A

Trypticase soy agar

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5
Q

Type of media that is basic nutrient media with extra nutrients that help grow fastidiouc (picky) bacteria

A

Enriched media

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6
Q

An enriched media that is very common and contains 5% sheep blood (blood cells in sheep blood are broken down quickly) in trypticase soy agar
Supports the growth of most pathogens

A

Blood agar plate (BAP)

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7
Q

An enriched media which blood is added to and then heated and used to grow organisms that cause STDs (Ex: Brucellosis)

A

Chocolate agar

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8
Q

Type of media that contains some type of substance that selects for one type of bacteria and inhibits other bacteria from growing

A

Selective media

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9
Q

A type of selective media that selects for Gram + bacteria

A

Phenylethel alcohol agar (PEA)

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10
Q

Very popular selective media that selects for GNROD only

Good to use with a lot of different bacteria

A

Macconkey agar (MAC)

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11
Q

Type of media that selects for a group of bacteria AND contains an indicator (visible change by the metabolic activity of the bacteria)

A

Differential media

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12
Q

Type of differential media that colonies change color if bacteria FERMENT LACTOSE
Pink = lactose fermentor
Clear = non-lactose fermentor

A

Macconkey (MAC)

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13
Q

Type of differential media that selects for Gram - enteric pathogens and inhibits all normal flora
Good for salmonella and shigella
Checks for sugar fermentors = salmon colored
Non sugar fermentors = blue/green

A

Hektoen-Enteric agar

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14
Q

A differential media that is used to classify bacteria according to their ability to ferment
Yellow = indicates fermentation
Red = no fermentation

A

Triple Sugar Iron (TSI)

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15
Q

A differential media that selects for genus Staphylococci and can be used to isolate Staph aureus
Yellow = organism can ferment mannitol
Red = non-pathogenic staph

A

Mannitol Salt Agar

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16
Q

Media type that is usually a broth and selects for growth of particular organisms and contain extra nutrients to allow bacteria to grow better
Used for fecal cultures

A

Selective broth media

17
Q

Broth that selects for and enriches enteric pathogens and inhibit normal bowel flora

A

Selenite

18
Q

Broth that suppresses coliform (gut) organisms

A

Tetrathionate broth

19
Q

Misc media that is a useful, all purpose broth and used to increase the number of organisms before they are plated onto a solid medium

A

Brain-heart infusion

20
Q

Misc broth media that determines motility of bacteria

A

Motility media

21
Q

Misc media that tests for detection of a bacteria’s ability to produce indole

A

Indole test media

22
Q

Creates partial hemolysis of blood agar

Narrow band of green, slimey discoloration around bacterial growth

A

Alpha

23
Q

Creates complete hemolysis of blood agar

Clear band around bacterial growth

A

Beta

24
Q

Created by non-hemolytic bacteria

No change of blood agar

A

Gamma

25
Q

Double zoned hemolysis

Not common

A

Delta

26
Q

Which quadrant of streaking is where most isolated colonies grow?

A

D

27
Q

What temp and how long should plates be incubated?

A

35-37* C, 24-48hr

28
Q

Sensitivity test that utilizes Mueller-Hinton media

Tests resistance to Abx

A

Kirby Bauer

29
Q

The larger the zone of inhibition, the more ____________ the bacteria is to the abx

A

susceptible

30
Q

R

A

Resistant

31
Q

I

A

Intermediate, abx may work

32
Q

S

A

Sensitive

Abx will kill bacteria

33
Q

Tests the lowest concentration of Abx at which a bacteria will not survive
Looks for the optimal effective Tx for the pt while avoiding overprescription

A

Minimum Inhibitory Concentration (MIC)

Clin Lab IV (5 decks)

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