Bacteria and Culturing Flashcards
Bacteria are between ____ microns in length
0.5-5
3 shapes of bacteria
- Cocci (round)
- Bacilli (rod)
- Spirilli (spiral)
Bacteria are anucleated, or ________
prokaryotic
Rigid, outer layer of a bacteria cell that gives it shape and protection
Cell wall
Most bacteria have cell walls, but what kind doesnt?
mycoplasma
Selectively permeable that controls what enters and exits the bacteria cell
cell membrane
Part of the bacteria cell that contains nutrients and organelles
Cytoplasm
Site of cellular division in a bacteria cell
Mesosomes
Gelatinous covering around the outside of the cell that helps the cell be antiphagocytic
Capsules
Part of a cell used for locomotion
flagella
Facilitates the adherence of bacteria to mucous membranes
Pilli/Fimbria
Makes the bacteria cell hard to kill by making it resistant to disinfection and helps the bacteria survive pasteurization
Ex: Clostridium piliforme
Spores/Endospores
A source of energy for bacteria that comes from organic molecules
Carbon
A source of energy for bacteria that is a biproduct of protein metabolism
Nitrogen
What percentage of a bacteria cell’s weight is water?
7-10%
What is the optimal pH for bacterial growth?
7-7.5
Optimal temperature for bacterial growth?
35-37 Celsius
95-98.6 Fahrenheit
Optimal temperature for pathogenic bacteria?
20-40 Celsius
68-104 Fahrenheit
How do bacteria reproduce?
Binary fission (asexually)
Bacteria that grow well in the presence of O2
Aerobic
Bacteria that grow well with lack of O2
Anaerobic
Bacteria that grow +/- O2
Facultative
Bacteria that need a certain amount of O2
Microaerophilic
Bacteria that grows best at 20-40 celsius with optimal growth at 35-37 celsius
Most pathogenic bacteria
Mesophilic
Cold loving bacteria that optimally grows at 0-30 Celsius
Psychrophiles
Hot loving bacteria that optimally grows at 40-80 Celsius
Survive pasteurization and hot springs
Thermophilic
5 Body fluids/organs that should be sterile
- blood
- urine
- CSF
- synovial fluids
- lungs
Good bacteria that produce vitamins and prevent dz
Normal Flora
Normal flora can be found in 4 places
- GI tract
- upper respiratory tract
- skin
- anterior urethra
Normal flora compete with pathogenic organisms for nutrients and space and produce toxins that are harmful to pathogenic microorganisms
Microbial Antagonists
Pathogenic bacteria that normally don’t cause dz but can under certain circumstances
Ex: E. Coli
Opportunistic
Pathogenic bacteria that always cause dz
NOT normal flora
Obligate
Probiotic supplement given to help normal flora
Lactobacillus
Excreted by Gram + and Gram - bacteria that make the host sick while the bacteria is alive
Ex: Clostridium tetani, Clostridium botullini
Exotoxins
Excreted by Gram - bacteria that make the host sick when the bacteria dies
Less antigenic
Ex: E. coli, Shigella, Salmonella
Endotoxins
Route of bacterial transmission where there is direct contact with the infection’s carrier
Direct
Route of bacterial transmission where it can be aerosolized and requires no physical contact
Usually through resp system or ingestion of contaminated food/H2O
Indirect
Route of transmission by way of an inanimate objects (pen, table, Sxical equipment)
Fomites
Transmission by way of an insect
Vector
Route of transmission by way of Non-GI infex
Ex: blood transfusion
Parenteral
MM produces _________ which traps microorganisms
mucous
3 phagocytic blood cells
- Monocytes/Macrophages
- Eos
- Neutros
2 immune responses
Innate and Adaptive
You should collect samples ________
aseptically
Material swabbed from a superficial body surface yield a ________ _______ bacteria culture
mixed growth
Material swabbed from body orifices are frequently contaminated with __________ ___________
normal flora
Most useful collection
aspirates from aseptically prepared sites
When should you collect a sample from a deceased animal or on Abx?
ASAP
Tissue samples should be at least
3 sq. cm.
Body fluid samples should be at least
1 mL
You should send ______ samples of urine
sterile (not free catch)
The easier, preferred method to obtain sterile urine
cystocentesis
Blood is best collected at the spike of a
F+
2 fluids that should be collected aseptically
CSF and synovial fluid