Bacteria and Culturing Flashcards

1
Q

Bacteria are between ____ microns in length

A

0.5-5

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2
Q

3 shapes of bacteria

A
  1. Cocci (round)
  2. Bacilli (rod)
  3. Spirilli (spiral)
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3
Q

Bacteria are anucleated, or ________

A

prokaryotic

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4
Q

Rigid, outer layer of a bacteria cell that gives it shape and protection

A

Cell wall

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5
Q

Most bacteria have cell walls, but what kind doesnt?

A

mycoplasma

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6
Q

Selectively permeable that controls what enters and exits the bacteria cell

A

cell membrane

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7
Q

Part of the bacteria cell that contains nutrients and organelles

A

Cytoplasm

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8
Q

Site of cellular division in a bacteria cell

A

Mesosomes

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9
Q

Gelatinous covering around the outside of the cell that helps the cell be antiphagocytic

A

Capsules

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10
Q

Part of a cell used for locomotion

A

flagella

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11
Q

Facilitates the adherence of bacteria to mucous membranes

A

Pilli/Fimbria

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12
Q

Makes the bacteria cell hard to kill by making it resistant to disinfection and helps the bacteria survive pasteurization
Ex: Clostridium piliforme

A

Spores/Endospores

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13
Q

A source of energy for bacteria that comes from organic molecules

A

Carbon

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14
Q

A source of energy for bacteria that is a biproduct of protein metabolism

A

Nitrogen

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15
Q

What percentage of a bacteria cell’s weight is water?

A

7-10%

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16
Q

What is the optimal pH for bacterial growth?

A

7-7.5

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17
Q

Optimal temperature for bacterial growth?

A

35-37 Celsius

95-98.6 Fahrenheit

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18
Q

Optimal temperature for pathogenic bacteria?

A

20-40 Celsius

68-104 Fahrenheit

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19
Q

How do bacteria reproduce?

A

Binary fission (asexually)

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20
Q

Bacteria that grow well in the presence of O2

A

Aerobic

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21
Q

Bacteria that grow well with lack of O2

A

Anaerobic

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22
Q

Bacteria that grow +/- O2

A

Facultative

23
Q

Bacteria that need a certain amount of O2

A

Microaerophilic

24
Q

Bacteria that grows best at 20-40 celsius with optimal growth at 35-37 celsius
Most pathogenic bacteria

A

Mesophilic

25
Q

Cold loving bacteria that optimally grows at 0-30 Celsius

A

Psychrophiles

26
Q

Hot loving bacteria that optimally grows at 40-80 Celsius

Survive pasteurization and hot springs

A

Thermophilic

27
Q

5 Body fluids/organs that should be sterile

A
  1. blood
  2. urine
  3. CSF
  4. synovial fluids
  5. lungs
28
Q

Good bacteria that produce vitamins and prevent dz

A

Normal Flora

29
Q

Normal flora can be found in 4 places

A
  1. GI tract
  2. upper respiratory tract
  3. skin
  4. anterior urethra
30
Q

Normal flora compete with pathogenic organisms for nutrients and space and produce toxins that are harmful to pathogenic microorganisms

A

Microbial Antagonists

31
Q

Pathogenic bacteria that normally don’t cause dz but can under certain circumstances
Ex: E. Coli

A

Opportunistic

32
Q

Pathogenic bacteria that always cause dz

NOT normal flora

33
Q

Probiotic supplement given to help normal flora

A

Lactobacillus

34
Q

Excreted by Gram + and Gram - bacteria that make the host sick while the bacteria is alive
Ex: Clostridium tetani, Clostridium botullini

35
Q

Excreted by Gram - bacteria that make the host sick when the bacteria dies
Less antigenic
Ex: E. coli, Shigella, Salmonella

A

Endotoxins

36
Q

Route of bacterial transmission where there is direct contact with the infection’s carrier

37
Q

Route of bacterial transmission where it can be aerosolized and requires no physical contact
Usually through resp system or ingestion of contaminated food/H2O

38
Q

Route of transmission by way of an inanimate objects (pen, table, Sxical equipment)

39
Q

Transmission by way of an insect

40
Q

Route of transmission by way of Non-GI infex

Ex: blood transfusion

A

Parenteral

41
Q

MM produces _________ which traps microorganisms

42
Q

3 phagocytic blood cells

A
  1. Monocytes/Macrophages
  2. Eos
  3. Neutros
43
Q

2 immune responses

A

Innate and Adaptive

44
Q

You should collect samples ________

A

aseptically

45
Q

Material swabbed from a superficial body surface yield a ________ _______ bacteria culture

A

mixed growth

46
Q

Material swabbed from body orifices are frequently contaminated with __________ ___________

A

normal flora

47
Q

Most useful collection

A

aspirates from aseptically prepared sites

48
Q

When should you collect a sample from a deceased animal or on Abx?

49
Q

Tissue samples should be at least

50
Q

Body fluid samples should be at least

51
Q

You should send ______ samples of urine

A

sterile (not free catch)

52
Q

The easier, preferred method to obtain sterile urine

A

cystocentesis

53
Q

Blood is best collected at the spike of a

54
Q

2 fluids that should be collected aseptically

A

CSF and synovial fluid