Culture Requirements and Stains

Question 1

H. Influenza

Answer 1

Chocolate agar
factor V(NAD) , factor X (hematin)

Question 2

Thayer Martin. What are the three components?

Answer 2

VPN ( vancomycin, Polymyxin, Nystatin)

Neisseria Gonorrhea, Meningitidis

Question 3

Pertussis

Answer 3

Bordet Gengou (potato agar)

Question 4

Tellurite agar, Loffler medium

Answer 4

Diptheria

Question 5

Lowenstein jensen agar. but what test is used instead usually and why?

Answer 5

For M. Tuberculosis.

Acid fast staining is faster. But the culture is still done, takes two to four weeks to grow)

Question 6

Mycoplasma Pneumoniae

Answer 6

Eaton agar with Cholesterol

Question 7

Pink on MacConkey agar

Answer 7

Lactose fermenting enterics

Question 8

Ecoli is seen as a green metallic sheen colony on what medium?

Answer 8

EMB (eosin methylene blue)

Question 9

Charcoal yeast extract buffered with what two components? grows what organism?

Answer 9

Legionella
cysteine and iron
(also a urine test for legionella)

Question 10

Fungi

Answer 10

Sab’s a fun freakin guy

Sabouraud agar.

Question 11

Giemsa stain

Answer 11

Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium

Question 12

PAS

Answer 12

Whipple disease

Tropheryma whipplei

Question 13

Ziehl neelson (carbolfuchsin)

Answer 13

Acid fast stain. (mycobacterium (TB), nocardia)

Question 14

India Ink

Answer 14

Cryptococcus neoformans (mucicarmine can stain this red also)

Question 15

Silver stain

Answer 15

Legionella, H. pylori, fungi (pneumocystis)